論文 - 詳細
| RRC ID | 39990 |
|---|---|
| 著者 | Stil A, Drapeau P. |
| タイトル | Neuronal labeling patterns in the spinal cord of adult transgenic Zebrafish. |
| ジャーナル | Dev Neurobiol |
| Abstract |
We describe neuronal patterns in the spinal cord of adult zebrafish. We studied the distribution of cells and processes in the three spinal regions reported in the literature: the 8th vertebra used as a transection injury site, the 15th vertebra mainly used for motor cell recordings and also for crush injury, and the 24th vertebra used to record motor nerve activity. We used well-known transgenic lines in which expression of green fluorescent protein (GFP) is driven by promoters to hb9 and isl1 in motoneurons, alx/chx10 and evx1 interneurons, ngn1 in sensory neurons and olig2 in oligodendrocytes, as well as antibodies for neurons (HuC/D, NF and SV2) and glia (GFAP). In isl1:GFP fish, GFP-positive processes are retained in the upper part of ventral horns and two subsets of cell bodies are observed. The pattern of the transgene in hb9:GFP adults is more diffuse and fibers are present broadly through the adult spinal cord. In alx/chx10 and evx1 lines we respectively observed two and three different GFP-positive populations. Finally, the ngn1:GFP transgene identifies dorsal root ganglion and some cells in dorsal horns. Interestingly some GFP positive fibers in ngn1:GFP fish are located around Mauthner axons and their density seems to be related to a rostrocaudal gradient. Many other cell types have been described in embryos and need to be studied in adults. Our findings provide a reference for further studies on spinal cytoarchitecture. Combined with physiological, histological and pathological/traumatic approaches, these studies will help clarify the operation of spinal locomotor circuits of adult zebrafish. |
| 巻・号 | 76(6) |
| ページ | 642-60 |
| 公開日 | 2016-6-1 |
| DOI | 10.1002/dneu.22350 |
| PMID | 26408263 |
| MeSH | Animals Animals, Genetically Modified Basic Helix-Loop-Helix Transcription Factors / genetics Basic Helix-Loop-Helix Transcription Factors / metabolism ELAV-Like Protein 3 / metabolism Embryo, Nonmammalian Gene Expression Regulation, Developmental / genetics Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism LIM-Homeodomain Proteins / genetics LIM-Homeodomain Proteins / metabolism Microscopy, Confocal Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism Neurons / metabolism* Spinal Cord / cytology* Transcription Factors / genetics Transcription Factors / metabolism Zebrafish Zebrafish Proteins / genetics Zebrafish Proteins / metabolism* |
| IF | 3.935 |
| 引用数 | 8 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY NEUROSCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(chx10:GFP) |