論文 - 詳細
| RRC ID | 41746 |
|---|---|
| 著者 | Ishida K, Nagahara H, Kogiso T, Aso T, Hayashi N, Akaike T. |
| タイトル | Cell adhesion aside from integrin system can abrogate anoikis in rat liver cells by down-regulation of FasL expression, not by activation of PI-3K/Akt and ERK signaling pathway. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Epithelial cells require contact with extracellular matrix (ECM) to inhibit detachment-induced apoptosis (anoikis). The ERK and PI-3K/Akt signaling pathways have been identified to inhibit anoikis. We present here a different story. An adult rat liver cell line, ARLJ301-3, underwent apoptosis within 4h under suspension conditions even with active forms of Akt and ERK1/2. Once ARLJ301-3 cells are plated on tissue culture plates coated with synthetic polymer, such as poly-(N-p-vinyl benzyl-O-beta-D-galactopyranosyl-D-gluconamide) (PVLA), poly-L-lysine or polystyrene, instead of functional ECM such as fibronectin, they could survive and proliferate without activation of Akt and ERK1/2. The expression of Fas receptor ligand (FasL) is specifically detected in cells under suspension conditions or treated with cytochalasin-D. We present here the first report that FasL expression is up-regulated by the cytoskeletal disruption directed by cytochalasin-D treatment or cell detachment from ECM. |
| 巻・号 | 300(1) |
| ページ | 201-8 |
| 公開日 | 2003-1-3 |
| DOI | 10.1016/s0006-291x(02)02790-0 |
| PII | S0006291X02027900 |
| PMID | 12480544 |
| MeSH | Animals Apoptosis / physiology* Cell Adhesion / physiology* Cell Line Cytochalasin D / pharmacology Cytoskeleton / drug effects Cytoskeleton / metabolism Down-Regulation Fas Ligand Protein Hepatocytes / cytology* Hepatocytes / drug effects Hepatocytes / metabolism* Integrins / physiology Membrane Glycoproteins / genetics* Mitogen-Activated Protein Kinase 1 / metabolism Mitogen-Activated Protein Kinase 3 Mitogen-Activated Protein Kinases / metabolism Phosphatidylinositol 3-Kinases / metabolism Protein Serine-Threonine Kinases* Proto-Oncogene Proteins / metabolism Proto-Oncogene Proteins c-akt Rats Signal Transduction |
| IF | 2.985 |
| 引用数 | 16 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ARLJ301-3(RCB0447) |