| RRC ID |
41746
|
| 著者 |
Ishida K, Nagahara H, Kogiso T, Aso T, Hayashi N, Akaike T.
|
| タイトル |
Cell adhesion aside from integrin system can abrogate anoikis in rat liver cells by down-regulation of FasL expression, not by activation of PI-3K/Akt and ERK signaling pathway.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Epithelial cells require contact with extracellular matrix (ECM) to inhibit detachment-induced apoptosis (anoikis). The ERK and PI-3K/Akt signaling pathways have been identified to inhibit anoikis. We present here a different story. An adult rat liver cell line, ARLJ301-3, underwent apoptosis within 4h under suspension conditions even with active forms of Akt and ERK1/2. Once ARLJ301-3 cells are plated on tissue culture plates coated with synthetic polymer, such as poly-(N-p-vinyl benzyl-O-beta-D-galactopyranosyl-D-gluconamide) (PVLA), poly-L-lysine or polystyrene, instead of functional ECM such as fibronectin, they could survive and proliferate without activation of Akt and ERK1/2. The expression of Fas receptor ligand (FasL) is specifically detected in cells under suspension conditions or treated with cytochalasin-D. We present here the first report that FasL expression is up-regulated by the cytoskeletal disruption directed by cytochalasin-D treatment or cell detachment from ECM.
|
| 巻・号 |
300(1)
|
| ページ |
201-8
|
| 公開日 |
2003-1-3
|
| DOI |
10.1016/s0006-291x(02)02790-0
|
| PII |
S0006291X02027900
|
| PMID |
12480544
|
| MeSH |
Animals
Apoptosis / physiology*
Cell Adhesion / physiology*
Cell Line
Cytochalasin D / pharmacology
Cytoskeleton / drug effects
Cytoskeleton / metabolism
Down-Regulation
Fas Ligand Protein
Hepatocytes / cytology*
Hepatocytes / drug effects
Hepatocytes / metabolism*
Integrins / physiology
Membrane Glycoproteins / genetics*
Mitogen-Activated Protein Kinase 1 / metabolism
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinases / metabolism
Phosphatidylinositol 3-Kinases / metabolism
Protein Serine-Threonine Kinases*
Proto-Oncogene Proteins / metabolism
Proto-Oncogene Proteins c-akt
Rats
Signal Transduction
|
| IF |
2.985
|
| 引用数 |
16
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
ARLJ301-3(RCB0447) |
