RRC ID 4293
Author Shakir MA, Jiang K, Struckhoff EC, Demarco RS, Patel FB, Soto MC, Lundquist EA.
Title The Arp2/3 activators WAVE and WASP have distinct genetic interactions with Rac GTPases in Caenorhabditis elegans axon guidance.
Journal Genetics
Abstract In the developing nervous system, axons are guided to their targets by the growth cone. Lamellipodial and filopodial protrusions from the growth cone underlie motility and guidance. Many molecules that control lamellipodia and filopodia formation, actin organization, and axon guidance have been identified, but it remains unclear how these molecules act together to control these events. Experiments are described here that indicate that, in Caenorhabditis elegans, two WH2-domain-containing activators of the Arp2/3 complex, WVE-1/WAVE and WSP-1/WASP, act redundantly in axon guidance and that GEX-2/Sra-1 and GEX-3/Kette, molecules that control WAVE activity, might act in both pathways. WAVE activity is controlled by Rac GTPases, and data are presented here that suggest WVE-1/WAVE and CED-10/Rac act in parallel to a pathway containing WSP-1/WASP and MIG-2/RhoG. Furthermore, results here show that the CED-10/WVE-1 and MIG-2/WSP-1 pathways act in parallel to two other molecules known to control lamellipodia and filopodia and actin organization, UNC-115/abLIM and UNC-34/Enabled. These results indicate that at least three actin-modulating pathways act in parallel to control actin dynamics and lamellipodia and filopodia formation during axon guidance (WASP-WAVE, UNC-115/abLIM, and UNC-34/Enabled).
Volume 179(4)
Pages 1957-71
Published 2008-8-1
DOI 10.1534/genetics.108.088963
PII genetics.108.088963
PMID 18689885
PMC PMC2516072
MeSH Actin-Related Protein 2 / genetics Actin-Related Protein 2 / metabolism Actin-Related Protein 2-3 Complex / genetics Actin-Related Protein 2-3 Complex / metabolism* Actin-Related Protein 3 / genetics Actin-Related Protein 3 / metabolism Animals Axons / metabolism Axons / physiology* Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Cell Movement Microfilament Proteins / genetics Microfilament Proteins / metabolism Mutation Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism Signal Transduction rac GTP-Binding Proteins / genetics rac GTP-Binding Proteins / metabolism*
IF 4.015
Times Cited 45
C.elegans tm597