論文 - 詳細
| RRC ID | 4299 |
|---|---|
| 著者 | Song MH, Aravind L, Müller-Reichert T, O'Connell KF. |
| タイトル | The conserved protein SZY-20 opposes the Plk4-related kinase ZYG-1 to limit centrosome size. |
| ジャーナル | Dev Cell |
| Abstract |
Microtubules are organized by the centrosome, a dynamic organelle that exhibits changes in both size and number during the cell cycle. Here we show that SZY-20, a putative RNA-binding protein, plays a critical role in limiting centrosome size in C. elegans. SZY-20 localizes in part to centrosomes and in its absence centrosomes possess increased levels of centriolar and pericentriolar components including gamma-tubulin and the centriole duplication factors ZYG-1 and SPD-2. These enlarged centrosomes possess normal centrioles, nucleate more microtubules, and fail to properly direct a number of microtubule-dependent processes. Depletion of ZYG-1 restores normal centrosome size and function to szy-20 mutants, whereas loss of szy-20 suppresses the centrosome duplication defects in both zyg-1 and spd-2 mutants. Our results describe a pathway that determines centrosome size and implicate centriole duplication factors in this process. |
| 巻・号 | 15(6) |
| ページ | 901-12 |
| 公開日 | 2008-12-1 |
| DOI | 10.1016/j.devcel.2008.09.018 |
| PII | S1534-5807(08)00400-0 |
| PMID | 19081077 |
| PMC | PMC2829447 |
| MeSH | Alleles Animals Caenorhabditis elegans Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* Cell Nucleus / metabolism Centrioles / ultrastructure Centrosome / ultrastructure* Gene Expression Regulation, Developmental* Microtubules / metabolism Models, Biological Models, Genetic Mutation Protein Kinases / chemistry Protein Kinases / metabolism Protein Kinases / physiology* Protein Serine-Threonine Kinases / metabolism* RNA-Binding Proteins / metabolism RNA-Binding Proteins / physiology* |
| IF | 10.092 |
| 引用数 | 35 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| 線虫 | tm1997 |