RRC ID 43690
Author Inoue Y, Hasegawa S, Yamada T, Date Y, Mizutani H, Nakata S, Matsunaga K, Akamatsu H.
Title Bimodal effect of retinoic acid on melanocyte differentiation identified by time-dependent analysis.
Journal Pigment Cell Melanoma Res
Abstract Retinoic acid (RA) is considered to control melanocytes; however, its precise mechanism remains unclear because of a bimodal effect, which promotes or inhibits melanin synthesis depending on the cell type, culture condition of melanocytes and skin conditions. In this study, we examined the effects of RA throughout each stage of differentiation of melanocytes using a mouse embryonic stem cell culture system to induce melanocytes. The results showed that RA has significantly different effects depending on the stage of differentiation of melanocytes. More specifically, RA promoted differentiation in earlier stages, wherein embryonic stem cells became melanoblasts via neural crest cells, and inhibited differentiation in later stages, wherein melanoblasts became melanocytes. It was revealed for the first time that melanocytes show markedly different reactions to RA depending on the stage of differentiation.
Volume 25(3)
Pages 299-311
Published 2012-5
DOI 10.1111/j.1755-148X.2012.00988.x
PMID 22364180
MeSH Animals Cell Differentiation / drug effects* Cell Differentiation / genetics Cells, Cultured Drug Evaluation, Preclinical Gene Expression Profiling Gene Expression Regulation / drug effects Humans Melanins / biosynthesis Melanocytes / drug effects* Melanocytes / metabolism Melanocytes / physiology Melanocytes / ultrastructure Mice Mice, Inbred C57BL Real-Time Polymerase Chain Reaction / methods Signal Transduction / drug effects Signal Transduction / genetics Signal Transduction / physiology Stem Cells Time Factors Tretinoin / pharmacology*
IF 6.115
Times Cited 8
Human and Animal Cells