論文 - 詳細
| RRC ID | 43835 |
|---|---|
| 著者 | Hazawa M, Hosokawa Y, Monzen S, Yoshino H, Kashiwakura I. |
| タイトル | Regulation of DNA damage response and cell cycle in radiation-resistant HL60 myeloid leukemia cells. |
| ジャーナル | Oncol Rep |
| Abstract |
The acquisition of resistance to radiation has been a matter of concern in clinical cases. However, mechanisms underlying the acquisition of radiation resistance are yet to be elucidated. We established a radiation-resistant strain (Res-HL60 cells) from HL60 leukemic cells and investigated their response to radiation. Res-HL60 cells not only proliferated on the fifth day after radiation but also had a high survival rate in a clonogenic assay. Although Chk1 was activated in HL60 cells after irradiation, the expression levels of Chk1 in Res-HL60 cells decreased. There were few differences between the two cell lines with regard to Chk2 activity. Res-HL60 cells show prolonged G2/M arrest and an early decrease in the extent of DNA damage. However, inhibitors against ATM/ATR and DNA-dependent protein kinase (DNA-PK) both abrogated the radiation resistance capacity of the cells. These results reveal that radiation-resistant strains have a high repair capacity, and inhibition of the repair system at an early stage is an effective strategy in the second round of radiation therapy. |
| 巻・号 | 28(1) |
| ページ | 55-61 |
| 公開日 | 2012-7-1 |
| DOI | 10.3892/or.2012.1771 |
| PMID | 22576796 |
| MeSH | Ataxia Telangiectasia Mutated Proteins Caffeine / pharmacology Caspase 3 / metabolism Cell Cycle Proteins / antagonists & inhibitors Cell Cycle Proteins / metabolism Cell Shape / radiation effects Cell Size Cell Survival / radiation effects Checkpoint Kinase 1 Chromones / pharmacology DNA Fragmentation* DNA Repair / drug effects DNA-Activated Protein Kinase / antagonists & inhibitors DNA-Activated Protein Kinase / metabolism DNA-Binding Proteins / antagonists & inhibitors DNA-Binding Proteins / metabolism Gene Expression / radiation effects HL-60 Cells Histones / metabolism Humans Leukemia, Myeloid M Phase Cell Cycle Checkpoints / radiation effects* Morpholines / pharmacology Protein Kinases / genetics Protein Kinases / metabolism Protein Serine-Threonine Kinases / antagonists & inhibitors Protein Serine-Threonine Kinases / metabolism Radiation Tolerance* Tumor Suppressor Proteins / antagonists & inhibitors Tumor Suppressor Proteins / metabolism |
| IF | 3.417 |
| 引用数 | 13 |
| WOS 分野 | ONCOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | |