| RRC ID |
44125
|
| 著者 |
Wang H, Li W, Jin X, Cui S, Zhao L.
|
| タイトル |
LIM and SH3 protein 1, a promoter of cell proliferation and migration, is a novel independent prognostic indicator in hepatocellular carcinoma.
|
| ジャーナル |
Eur J Cancer
|
| Abstract |
LIM and SH3 protein 1 (LASP-1) plays a significant role in the formation of several malignant tumours. However, the biological and clinical significances of LASP-1 in hepatocellular carcinoma (HCC) remain largely unknown. Using immunohistochemistry, we analysed LASP-1 expression in 144 clinicopathologically characterised HCC cases. Using gene and transfection and RNA interference, we investigated the effects of LASP-1 overexpression and depletion on tumour cellular behaviour in vitro. LASP-1 expression was detected in not only cytoplasm and but also nucleus of HCC and liver cells. The positive rates of both cytosolic and nuclear LASP-1 expression in HCC were higher than adjacent non-tumourous tissues. Statistical analysis showed that heterogeneous LASP-1 expression is associated with hepatitis B surface antigen (HBsAg) and serum alpha-fetoprotein (AFP) level of HCC patients. A significant trend was identified between cytosolic LASP-1 overexpression in HCC and worsening clinical prognosis. Multivariate survival analysis showed that cytosolic LASP-1 expression was recognised as an independent prognostic factor of patient's survival. In vitro study showed LASP-1 promoted cell proliferation and migration, and resulted in aggressive phenotypes of cancer cells. LASP-1 is a valuable marker of HCC progression. High cytosolic LASP-1 expression is associated with poor overall survival in HCC patients.
|
| 巻・号 |
49(4)
|
| ページ |
974-83
|
| 公開日 |
2013-3-1
|
| DOI |
10.1016/j.ejca.2012.09.032
|
| PII |
S0959-8049(12)00783-6
|
| PMID |
23084841
|
| MeSH |
Adaptor Proteins, Signal Transducing / antagonists & inhibitors
Adaptor Proteins, Signal Transducing / genetics
Adaptor Proteins, Signal Transducing / metabolism*
Apoptosis
Blotting, Western
Carcinoma, Hepatocellular / metabolism
Carcinoma, Hepatocellular / mortality
Carcinoma, Hepatocellular / pathology*
Case-Control Studies
Cell Adhesion
Cell Cycle
Cell Movement*
Cell Nucleus
Cell Proliferation*
Cells, Cultured
Cytoplasm
Cytoskeletal Proteins / antagonists & inhibitors
Cytoskeletal Proteins / genetics
Cytoskeletal Proteins / metabolism*
Female
Follow-Up Studies
Gene Expression Regulation, Neoplastic
Hepatitis B Surface Antigens / metabolism
Hepatocytes
Humans
Immunoenzyme Techniques
LIM Domain Proteins / antagonists & inhibitors
LIM Domain Proteins / genetics
LIM Domain Proteins / metabolism*
Liver Neoplasms / metabolism
Liver Neoplasms / mortality
Liver Neoplasms / pathology*
Male
Middle Aged
Neoplasm Staging
Prognosis
RNA, Small Interfering / genetics
Survival Rate
|
| IF |
7.275
|
| 引用数 |
36
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HuH-7(RCB1366) |
