RRC ID 44304
Author Stergiou L, Bauer M, Mair W, Bausch-Fluck D, Drayman N, Wollscheid B, Oppenheim A, Pelkmans L.
Title Integrin-mediated signaling induced by simian virus 40 leads to transient uncoupling of cortical actin and the plasma membrane.
Journal PLoS One
Abstract Simian Virus 40 (SV40) is a paradigm pathogen with multivalent binding sites for the sphingolipid GM1, via which it induces its endocytosis for infection. Here we report that SV40 also utilizes cell surface integrins to activate signaling networks required for infection, even in the absence of the previously implicated glycosphingolipids. We identify ILK, PDK1, the RhoGAP GRAF1 and RhoA as core nodes of the signaling network activated upon SV40 engagement of integrins. We show that integrin-mediated signaling through host SV40 engagement induces the de-phosphorylation of Ezrin leading to uncoupling of the plasma membrane and cortical actin. Our results provide functional evidence for a mechanism by which SV40 activates signal transduction in human epithelial cells via integrins in the context of clathrin-independent endocytosis.
Volume 8(2)
Pages e55799
Published 2013
DOI 10.1371/journal.pone.0055799
PII PONE-D-12-24737
PMID 23409046
PMC PMC3567119
MeSH Actins / metabolism* Animals Cell Adhesion / physiology Cell Line Cell Membrane / metabolism* Cytoskeletal Proteins / metabolism Epistasis, Genetic Gene Regulatory Networks Glycosphingolipids / metabolism Humans Integrins / metabolism* Membrane Glycoproteins / metabolism Phosphatidylinositol 3-Kinases / metabolism Phosphorylation Polyomavirus Infections / genetics Polyomavirus Infections / metabolism Protein Binding Protein Interaction Maps Protein-Serine-Threonine Kinases / metabolism Proteomics Proto-Oncogene Proteins c-akt Pyruvate Dehydrogenase (Acetyl-Transferring) Kinase RNA Interference Signal Transduction* Simian virus 40 / physiology* Virus Internalization rhoA GTP-Binding Protein / metabolism
IF 2.776
Times Cited 15
Human and Animal Cells