RRC ID 44526
Author Akiyama M, Ozaki K, Kawano T, Yamada O, Kawauchi K, Ida H, Yamada H.
Title Telomerase activation as a repair response to radiation-induced DNA damage in Y79 retinoblastoma cells.
Journal Cancer Lett.
Abstract The molecular mechanism of telomerase activation induced by ionizing radiation (IR) remains poorly understood. We demonstrate that DNA damage induced by IR at doses of 2-5 Gy triggers activation of Akt, predominant to that of protein phosphatase 2A (PP2A), resulting in human telomerase reverse transcriptase (hTERT) phosphorylation and increased telomerase activity in Y79 cells. DNA damage induced by IR at doses greater than 10 Gy might trigger PP2A activation, predominant to that of Akt, resulting in hTERT dephosphorylation and decreased telomerase activity. Our results suggest that differential activation of Akt and PP2A may be responsible for telomerase regulation.
Volume 340(1)
Pages 82-7
Published 2013-10-28
DOI 10.1016/j.canlet.2013.07.003
PII S0304-3835(13)00508-9
PMID 23850566
MeSH Androstadienes / pharmacology Apoptosis / radiation effects Cell Line, Tumor DNA Damage* DNA Repair* Enzyme Activation G2 Phase Cell Cycle Checkpoints / radiation effects Gene Expression Histones / metabolism Humans MAP Kinase Signaling System Okadaic Acid / pharmacology Phosphorylation Protein Processing, Post-Translational Retinoblastoma Telomerase / genetics Telomerase / metabolism* Tumor Suppressor Protein p53 / metabolism Wortmannin
IF 6.491
Times Cited 8
Human and Animal Cells