論文 - 詳細
| RRC ID | 45202 |
|---|---|
| 著者 | Shen Y, Pan X, Zhao H. |
| タイトル | The histone demethylase PHF8 is an oncogenic protein in human non-small cell lung cancer. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
PHF8 is a JmjC domain-containing protein and erases repressive histone marks including H4K20me1 and H3K9me1/2. It binds to H3K4me3, an active histone mark usually located at transcription start sites (TSSs), through its plant homeo-domain, and is thus recruited and enriched in gene promoters. PHF8 is involved in the development of several types of cancer, including leukemia, prostate cancer, and esophageal squamous cell carcinoma. Herein we report that PHF8 is an oncogenic protein in human non-small cell lung cancer (NSCLC). PHF8 is up-regulated in human NSCLC tissues, and high PHF8 expression predicts poor survival. Our in vitro and in vivo evidence demonstrate that PHF8 regulates lung cancer cell proliferation and cellular transformation. We found that PHF8 knockdown induces DNA damage and apoptosis in lung cancer cells. PHF8 promotes miR-21 expression in human lung cancer, and miR-21 knockdown blocks the effects of PHF8 on proliferation and apoptosis of lung cancer cells. In summary, PHF8 promotes lung cancer cell growth and survival by regulating miR-21. |
| 巻・号 | 451(1) |
| ページ | 119-25 |
| 公開日 | 2014-8-15 |
| DOI | 10.1016/j.bbrc.2014.07.076 |
| PII | S0006-291X(14)01318-7 |
| PMID | 25065740 |
| MeSH | Aged Animals Apoptosis / genetics Carcinoma, Non-Small-Cell Lung / enzymology* Carcinoma, Non-Small-Cell Lung / genetics Carcinoma, Non-Small-Cell Lung / mortality Carcinoma, Non-Small-Cell Lung / pathology Cell Proliferation Female Gene Expression Regulation, Neoplastic Gene Knockdown Techniques Histone Demethylases / genetics Histone Demethylases / metabolism* Humans Lung Neoplasms / enzymology* Lung Neoplasms / genetics Lung Neoplasms / mortality Lung Neoplasms / pathology Male Mice Mice, Nude MicroRNAs / genetics* Middle Aged Predictive Value of Tests Reference Values Transcription Factors / genetics Transcription Factors / metabolism* Xenograft Model Antitumor Assays |
| IF | 2.985 |
| 引用数 | 33 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | A549 RERF-LC-AI(RCB0444) |