論文 - 詳細
| RRC ID | 45545 |
|---|---|
| 著者 | Connolly AA, Sugioka K, Chuang CH, Lowry JB, Bowerman B. |
| タイトル | KLP-7 acts through the Ndc80 complex to limit pole number in C. elegans oocyte meiotic spindle assembly. |
| ジャーナル | J Cell Biol |
| Abstract |
During oocyte meiotic cell division in many animals, bipolar spindles assemble in the absence of centrosomes, but the mechanisms that restrict pole assembly to a bipolar state are unknown. We show that KLP-7, the single mitotic centromere-associated kinesin (MCAK)/kinesin-13 in Caenorhabditis elegans, is required for bipolar oocyte meiotic spindle assembly. In klp-7(-) mutants, extra microtubules accumulated, extra functional spindle poles assembled, and chromosomes frequently segregated as three distinct masses during meiosis I anaphase. Moreover, reducing KLP-7 function in monopolar klp-18(-) mutants often restored spindle bipolarity and chromosome segregation. MCAKs act at kinetochores to correct improper kinetochore-microtubule (k-MT) attachments, and depletion of the Ndc-80 kinetochore complex, which binds microtubules to mediate kinetochore attachment, restored bipolarity in klp-7(-) mutant oocytes. We propose a model in which KLP-7/MCAK regulates k-MT attachment and spindle tension to promote the coalescence of early spindle pole foci that produces a bipolar structure during the acentrosomal process of oocyte meiotic spindle assembly. |
| 巻・号 | 210(6) |
| ページ | 917-32 |
| 公開日 | 2015-9-14 |
| DOI | 10.1083/jcb.201412010 |
| PII | jcb.201412010 |
| PMID | 26370499 |
| PMC | PMC4576866 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Female Genotype Kinesins / genetics Kinesins / metabolism* Meiosis* Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism* Microtubules / metabolism Mutation Oocytes / metabolism* Phenotype Signal Transduction Spindle Poles / genetics Spindle Poles / metabolism* Time Factors |
| IF | 8.811 |
| 引用数 | 17 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2143 |