論文 - 詳細
| RRC ID | 45550 |
|---|---|
| 著者 | de Albuquerque BF, Placentino M, Ketting RF. |
| タイトル | Maternal piRNAs Are Essential for Germline Development following De Novo Establishment of Endo-siRNAs in Caenorhabditis elegans. |
| ジャーナル | Dev Cell |
| Abstract |
The Piwi-piRNA pathway represents a germline-specific transposon-defense system. C. elegans Piwi, prg-1, is a non-essential gene and triggers a secondary RNAi response that depends on mutator genes, endo-siRNAs (22G-RNAs), and the 22G-RNA-binding Argonaute protein HRDE-1. Interestingly, silencing of PRG-1 targets can become PRG-1 independent. This state, known as RNAe, is heritable and depends on mutator genes and HRDE-1. We studied how the transgenerational memory of RNAe and the piRNA pathway interact. We find that maternally provided PRG-1 is required for de novo establishment of 22G-RNA populations, especially those targeting transposons. Strikingly, attempts to re-establish 22G-RNAs in absence of both PRG-1 and RNAe memory result in severe germline proliferation defects. This is accompanied by a disturbed balance between gene-activating and -repressing 22G-RNA pathways. We propose a model in which CSR-1 prevents the loading of HRDE-1 and in which both PRG-1 and HRDE-1 help to keep mutator activity focused on the proper targets. |
| 巻・号 | 34(4) |
| ページ | 448-56 |
| 公開日 | 2015-8-24 |
| DOI | 10.1016/j.devcel.2015.07.010 |
| PII | S1534-5807(15)00461-X |
| PMID | 26279485 |
| MeSH | Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism DNA Transposable Elements / genetics Gene Silencing Genes, Helminth Germ Cells / growth & development* Germ Cells / metabolism Mutation / genetics RNA, Helminth / genetics RNA, Small Interfering / genetics RNA, Small Interfering / metabolism* Signal Transduction / genetics |
| IF | 10.092 |
| 引用数 | 37 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1021 |