論文 - 詳細
RRC ID | 45583 |
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著者 | Kim Y, Kostow N, Dernburg AF. |
タイトル | The Chromosome Axis Mediates Feedback Control of CHK-2 to Ensure Crossover Formation in C. elegans. |
ジャーナル | Dev Cell |
Abstract |
CHK-2 kinase is a master regulator of meiosis in C. elegans. Its activity is required for homolog pairing and synapsis and for double-strand break formation, but how it drives and coordinates these pathways to ensure crossover formation remains unknown. Here we show that CHK-2 promotes pairing and synapsis by phosphorylating a family of zinc finger proteins that bind to specialized regions on each chromosome known as pairing centers, priming their recruitment of the Polo-like kinase PLK-2. This knowledge enabled the development of a phospho-specific antibody as a tool to monitor CHK-2 activity. When either synapsis or crossover formation is impaired, CHK-2 activity is prolonged, and meiotic progression is delayed. We show that this common feedback circuit is mediated by interactions among a network of HORMA domain proteins within the chromosome axis and generates a graded signal. These findings reveal conserved regulatory mechanisms that ensure faithful meiotic chromosome segregation in diverse species. |
巻・号 | 35(2) |
ページ | 247-61 |
公開日 | 2015-10-26 |
DOI | 10.1016/j.devcel.2015.09.021 |
PII | S1534-5807(15)00622-X |
PMID | 26506311 |
PMC | PMC4624198 |
MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Checkpoint Kinase 2 / genetics* Checkpoint Kinase 2 / metabolism Chromosome Pairing / genetics Chromosome Segregation / genetics* Chromosomes / genetics Crossing Over, Genetic DNA Breaks, Double-Stranded Meiosis / genetics* Protein Serine-Threonine Kinases / genetics* Protein Serine-Threonine Kinases / metabolism |
IF | 10.092 |
引用数 | 19 |
WOS 分野 | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
リソース情報 | |
線虫 | tm1395 tm611 tm3655 tm2543 |