RRC ID 45583
著者 Kim Y, Kostow N, Dernburg AF.
タイトル The Chromosome Axis Mediates Feedback Control of CHK-2 to Ensure Crossover Formation in C. elegans.
ジャーナル Dev Cell
Abstract CHK-2 kinase is a master regulator of meiosis in C. elegans. Its activity is required for homolog pairing and synapsis and for double-strand break formation, but how it drives and coordinates these pathways to ensure crossover formation remains unknown. Here we show that CHK-2 promotes pairing and synapsis by phosphorylating a family of zinc finger proteins that bind to specialized regions on each chromosome known as pairing centers, priming their recruitment of the Polo-like kinase PLK-2. This knowledge enabled the development of a phospho-specific antibody as a tool to monitor CHK-2 activity. When either synapsis or crossover formation is impaired, CHK-2 activity is prolonged, and meiotic progression is delayed. We show that this common feedback circuit is mediated by interactions among a network of HORMA domain proteins within the chromosome axis and generates a graded signal. These findings reveal conserved regulatory mechanisms that ensure faithful meiotic chromosome segregation in diverse species.
巻・号 35(2)
ページ 247-61
公開日 2015-10-26
DOI 10.1016/j.devcel.2015.09.021
PII S1534-5807(15)00622-X
PMID 26506311
PMC PMC4624198
MeSH Animals Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Checkpoint Kinase 2 / genetics* Checkpoint Kinase 2 / metabolism Chromosome Pairing / genetics Chromosome Segregation / genetics* Chromosomes / genetics Crossing Over, Genetic DNA Breaks, Double-Stranded Meiosis / genetics* Protein Serine-Threonine Kinases / genetics* Protein Serine-Threonine Kinases / metabolism
IF 10.092
引用数 19
WOS 分野 DEVELOPMENTAL BIOLOGY CELL BIOLOGY
リソース情報
線虫 tm1395 tm611 tm3655 tm2543