RRC ID 45947
著者 Chen YZ, Mapes J, Lee ES, Skeen-Gaar RR, Xue D.
タイトル Caspase-mediated activation of Caenorhabditis elegans CED-8 promotes apoptosis and phosphatidylserine externalization.
ジャーナル Nat Commun
Abstract During apoptosis, phosphatidylserine (PS), normally restricted to the inner leaflet of the plasma membrane, is exposed on the surface of apoptotic cells and serves as an 'eat-me' signal to trigger phagocytosis. It is poorly understood how PS exposure is activated in apoptotic cells. Here we report that CED-8, a Caenorhabditis elegans protein implicated in controlling the kinetics of apoptosis and a homologue of the XK family proteins, is a substrate of the CED-3 caspase. Cleavage of CED-8 by CED-3 activates its proapoptotic function and generates a carboxyl-terminal cleavage product, acCED-8, that promotes PS externalization in apoptotic cells and can induce ectopic PS exposure in living cells. Consistent with its role in promoting PS externalization in apoptotic cells, ced-8 is important for cell corpse engulfment in C. elegans. Our finding identifies a crucial link between caspase activation and PS externalization, which triggers phagocytosis of apoptotic cells.
巻・号 4
ページ 2726
公開日 2013-1-1
DOI 10.1038/ncomms3726
PII ncomms3726
PMID 24225442
PMC PMC3939056
MeSH Alleles Animals Animals, Genetically Modified Apoptosis* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism* Caspases / metabolism* Cell Membrane / metabolism Enzyme Activation Gene Expression Regulation, Developmental Green Fluorescent Proteins / metabolism Heat-Shock Proteins / metabolism Membrane Proteins / metabolism* Mutation Phagocytosis Phosphatidylserines / metabolism*
IF 12.121
引用数 41
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
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線虫 tm2078 tm3117