論文 - 詳細
| RRC ID | 45968 |
|---|---|
| 著者 | Ferreira HC, Towbin BD, Jegou T, Gasser SM. |
| タイトル | The shelterin protein POT-1 anchors Caenorhabditis elegans telomeres through SUN-1 at the nuclear periphery. |
| ジャーナル | J Cell Biol |
| Abstract |
Telomeres are specialized protein-DNA structures that protect chromosome ends. In budding yeast, telomeres form clusters at the nuclear periphery. By imaging telomeres in embryos of the metazoan Caenorhabditis elegans, we found that telomeres clustered only in strains that had activated an alternative telomere maintenance pathway (ALT). Moreover, as in yeast, the unclustered telomeres in wild-type embryos were located near the nuclear envelope (NE). This bias for perinuclear localization increased during embryogenesis and persisted in differentiated cells. Telomere position in early embryos required the NE protein SUN-1, the single-strand binding protein POT-1, and the small ubiquitin-like modifier (SUMO) ligase GEI-17. However, in postmitotic larval cells, none of these factors individually were required for telomere anchoring, which suggests that additional mechanisms anchor in late development. Importantly, targeted POT-1 was sufficient to anchor chromatin to the NE in a SUN-1-dependent manner, arguing that its effect at telomeres is direct. This high-resolution description of telomere position within C. elegans extends our understanding of telomere organization in eukaryotes. |
| 巻・号 | 203(5) |
| ページ | 727-35 |
| 公開日 | 2013-12-9 |
| DOI | 10.1083/jcb.201307181 |
| PII | jcb.201307181 |
| PMID | 24297748 |
| PMC | PMC3857485 |
| MeSH | Animals Caenorhabditis elegans / embryology Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / analysis Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* Cell Nucleus / metabolism Cell Nucleus / ultrastructure Chromosome Positioning DNA-Binding Proteins / analysis DNA-Binding Proteins / metabolism DNA-Binding Proteins / physiology* Embryo, Nonmammalian / metabolism Embryo, Nonmammalian / ultrastructure Embryonic Development / genetics Receptors, Cytoplasmic and Nuclear / analysis Receptors, Cytoplasmic and Nuclear / metabolism Receptors, Cytoplasmic and Nuclear / physiology* Sumoylation Telomere / metabolism* Telomere-Binding Proteins / analysis Telomere-Binding Proteins / metabolism Telomere-Binding Proteins / physiology Ubiquitin-Protein Ligases / metabolism Ubiquitin-Protein Ligases / physiology |
| IF | 8.811 |
| 引用数 | 21 |
| WOS 分野 | CELL BIOLOGY |
| リソース情報 | |
| 線虫 | tm1620 tm1400 |