Reference - Detail
| RRC ID | 46015 |
|---|---|
| Author | Aoki R, Yagami T, Sasakura H, Ogura K, Kajihara Y, Ibi M, Miyamae T, Nakamura F, Asakura T, Kanai Y, Misu Y, Iino Y, Ezcurra M, Schafer WR, Mori I, Goshima Y. |
| Title | A seven-transmembrane receptor that mediates avoidance response to dihydrocaffeic acid, a water-soluble repellent in Caenorhabditis elegans. |
| Journal | J Neurosci |
| Abstract |
The ability to detect harmful chemicals rapidly is essential for the survival of all animals. In Caenorhabditis elegans (C. elegans), repellents trigger an avoidance response, causing animals to move away from repellents. Dihydrocaffeic acid (DHCA) is a water-soluble repellent and nonflavonoid catecholic compound that can be found in plant products. Using a Xenopus laevis (X. laevis) oocyte expression system, we identified a candidate dihydrocaffeic acid receptor (DCAR), DCAR-1. DCAR-1 is a novel seven-transmembrane protein that is expressed in the ASH avoidance sensory neurons of C. elegans. dcar-1 mutant animals are defective in avoidance response to DHCA, and cell-specific expression of dcar-1 in the ASH neurons of dcar-1 mutant animals rescued the defect in avoidance response to DHCA. Our findings identify DCAR-1 as the first seven-transmembrane receptor required for avoidance of a water-soluble repellent, DHCA, in C. elegans. |
| Volume | 31(46) |
| Pages | 16603-10 |
| Published | 2011-11-16 |
| DOI | 10.1523/JNEUROSCI.4018-11.2011 |
| PII | 31/46/16603 |
| PMID | 22090488 |
| PMC | PMC6633322 |
| MeSH | 3,4-Dihydroxyphenylacetic Acid / pharmacology Analysis of Variance Animals Animals, Genetically Modified Behavior, Animal / drug effects Caenorhabditis elegans / physiology Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Caffeic Acids / pharmacology* Catechols / pharmacology Cloning, Molecular / methods Dose-Response Relationship, Drug Escape Reaction / drug effects* Escape Reaction / physiology Hydroxybenzoates Larva Luminescent Proteins / genetics Luminescent Proteins / metabolism Membrane Potentials / drug effects Membrane Potentials / genetics Microinjections / methods Models, Molecular Mutation / genetics Receptors, G-Protein-Coupled / genetics Receptors, G-Protein-Coupled / metabolism* Sensory Receptor Cells / drug effects Sensory Receptor Cells / physiology Xenopus |
| IF | 5.674 |
| Times Cited | 13 |
| WOS Category | NEUROSCIENCES |
| Resource | |
| C.elegans | tm2484 |