RRC ID 46247
Author Zhang P, Zhang H.
Title Autophagy modulates miRNA-mediated gene silencing and selectively degrades AIN-1/GW182 in C. elegans.
Journal EMBO Rep
Abstract MicroRNAs (miRNAs) post-transcriptionally repress gene expression via the miRNA-induced silencing complex (miRISC), which includes miRNA, Argonaute and a GW182 family member. Here we show that in Caenorhabditis elegans, miRNA-mediated gene silencing is modulated by macroautophagy, a lysosome-mediated degradation process. Loss of autophagy activity suppresses developmental defects caused by partially impaired silencing of miRNA targets including the let-7 family and lsy-6. The C. elegans GW182 homolog AIN-1 is itself selectively degraded by autophagy and colocalizes with the p62 homolog SQST-1 in autophagy mutants. Thus, autophagy activity modulates miRNA-mediated gene silencing and degrades a core miRISC component.
Volume 14(6)
Pages 568-76
Published 2013-6
DOI 10.1038/embor.2013.53
PII embor201353
PMID 23619095
PMC PMC3674441
MeSH Animals Autophagy* Caenorhabditis elegans / cytology Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Carrier Proteins / genetics* Carrier Proteins / metabolism Gene Expression Regulation, Developmental Larva / cytology Larva / genetics Larva / growth & development MicroRNAs / genetics* Mutation Proteolysis RNA Interference* RNA-Induced Silencing Complex / genetics ras Proteins / genetics ras Proteins / metabolism
IF 8.383
Times Cited 35
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
C.elegans tm3425 tm2508