RRC ID 46291
著者 Mohamed AM, Chin-Sang ID.
タイトル The C. elegans nck-1 gene encodes two isoforms and is required for neuronal guidance.
ジャーナル Dev Biol
Abstract The NCK adaptor proteins are composed entirely of SH3 and SH2 domains and serve as protein interaction bridges for several receptors during signal transduction events. Here we report the molecular and genetic analysis of the Caenorhabditis elegans nck-1 gene. C. elegans nck-1 encodes two isoforms: NCK-1A and a shorter isoform that lacks the first SH3 domain, NCK-1B. C. elegans nck-1 mutants exhibit defects in axon guidance and neuronal cell position, as well as defects in the excretory canal cell, gonad, and male mating. NCK-1 is broadly expressed in neurons and epithelial cells with NCK-1B being the most abundant isoform. NCK-1A and NCK-1B share a similar expression pattern in parts of the nervous system, but also have independent expression patterns in other tissues. Interestingly, NCK-1B is localized to the nuclei of many cells. Genetic rescue experiments show that NCK-1 functions cell autonomously and, in general, either NCK-1A or NCK-1B is sufficient to function in axon guidance. However, there appears to be specific roles for each isoform, for example NCK-1B is required for HSN cell migration while NCK-1A is required for efficient male mating. Genetic epistasis experiments show that NCK-1 functions redundantly with the LAR Receptor Tyrosine Phosphatase, PTP-3, and the Netrin receptor UNC-40.
巻・号 354(1)
ページ 55-66
公開日 2011-6-1
DOI 10.1016/j.ydbio.2011.03.019
PII S0012-1606(11)00183-7
PMID 21443870
MeSH Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / metabolism* Amino Acid Sequence Animals Animals, Genetically Modified Axons / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Movement Cell Nucleus / metabolism Cytoplasm / metabolism Female Gene Expression Profiling Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Male Microscopy, Confocal Molecular Sequence Data Mutation Neurogenesis* Neurons / cytology Neurons / metabolism* Oncogene Proteins / genetics Oncogene Proteins / metabolism* Protein Isoforms / genetics Protein Isoforms / metabolism Sequence Homology, Amino Acid
IF 2.896
引用数 4
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
線虫 tm1645 tm2561