RRC ID 46566
Author Back P, Matthijssens F, Vlaeminck C, Braeckman BP, Vanfleteren JR.
Title Effects of sod gene overexpression and deletion mutation on the expression profiles of reporter genes of major detoxification pathways in Caenorhabditis elegans.
Journal Exp Gerontol
Abstract Reactive oxygen species have long been considered a major cause of aging. However, previous work showed that loss of superoxide dismutase (SOD) only weakly affects lifespan of Caenorhabditis elegans. Here, we examined the impact of sod gene deletion and overexpression on the mRNA levels of the remaining sod genes and other detoxification genes. We detected no compensatory upregulation of other sod genes in any of the sod deletion mutants in both wild-type and daf-2(m577) genetic backgrounds when L4 larvae were shifted from 17 to 24 degrees C, and harvested as young adults. Elimination of MnSOD increased transcription of SKN-1 regulated genes and reduced transcription of multiple DAF-16 targets. Loss of the major Cu/ZnSOD isoform SOD-1 caused enhanced expression of subsets of both SKN-1 and DAF-16 targets when the animals were grown continuously at 24 degrees C, and strong overexpression of sod-1 induced a compensatory decrease in all tested SKN-1 regulated gst genes. When combined, these results suggest that low cytosolic SOD may activate SKN-1 signaling, whereas high levels may be repressive. Overall, our results suggest that sod gene manipulation causes complex, combinatorial regulation of expression of individual targets of stress sensitive transcription factors.
Volume 45(7-8)
Pages 603-10
Published 2010-8-1
DOI 10.1016/j.exger.2010.01.014
PII S0531-5565(10)00048-3
PMID 20096764
MeSH Aging / genetics Aging / metabolism Animals Animals, Genetically Modified Binding Sites / genetics Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism DNA-Binding Proteins / metabolism Forkhead Transcription Factors GATA Transcription Factors / metabolism Gene Deletion Gene Expression Profiling Gene Expression Regulation, Developmental Genes, Helminth* Genes, Reporter Inactivation, Metabolic / genetics Inactivation, Metabolic / physiology Longevity / genetics Longevity / physiology Mutation Oxidative Stress Promoter Regions, Genetic RNA, Helminth / genetics RNA, Helminth / metabolism RNA, Messenger / genetics RNA, Messenger / metabolism Signal Transduction Superoxide Dismutase / genetics* Superoxide Dismutase / metabolism Superoxide Dismutase-1 Transcription Factors / metabolism
IF 3.376
Times Cited 33
C.elegans NA