| RRC ID |
46566
|
| 著者 |
Back P, Matthijssens F, Vlaeminck C, Braeckman BP, Vanfleteren JR.
|
| タイトル |
Effects of sod gene overexpression and deletion mutation on the expression profiles of reporter genes of major detoxification pathways in Caenorhabditis elegans.
|
| ジャーナル |
Exp Gerontol
|
| Abstract |
Reactive oxygen species have long been considered a major cause of aging. However, previous work showed that loss of superoxide dismutase (SOD) only weakly affects lifespan of Caenorhabditis elegans. Here, we examined the impact of sod gene deletion and overexpression on the mRNA levels of the remaining sod genes and other detoxification genes. We detected no compensatory upregulation of other sod genes in any of the sod deletion mutants in both wild-type and daf-2(m577) genetic backgrounds when L4 larvae were shifted from 17 to 24 degrees C, and harvested as young adults. Elimination of MnSOD increased transcription of SKN-1 regulated genes and reduced transcription of multiple DAF-16 targets. Loss of the major Cu/ZnSOD isoform SOD-1 caused enhanced expression of subsets of both SKN-1 and DAF-16 targets when the animals were grown continuously at 24 degrees C, and strong overexpression of sod-1 induced a compensatory decrease in all tested SKN-1 regulated gst genes. When combined, these results suggest that low cytosolic SOD may activate SKN-1 signaling, whereas high levels may be repressive. Overall, our results suggest that sod gene manipulation causes complex, combinatorial regulation of expression of individual targets of stress sensitive transcription factors.
|
| 巻・号 |
45(7-8)
|
| ページ |
603-10
|
| 公開日 |
2010-8-1
|
| DOI |
10.1016/j.exger.2010.01.014
|
| PII |
S0531-5565(10)00048-3
|
| PMID |
20096764
|
| MeSH |
Aging / genetics
Aging / metabolism
Animals
Animals, Genetically Modified
Binding Sites / genetics
Caenorhabditis elegans / enzymology*
Caenorhabditis elegans / genetics*
Caenorhabditis elegans / growth & development
Caenorhabditis elegans Proteins / genetics*
Caenorhabditis elegans Proteins / metabolism
DNA-Binding Proteins / metabolism
Forkhead Transcription Factors
GATA Transcription Factors / metabolism
Gene Deletion
Gene Expression Profiling
Gene Expression Regulation, Developmental
Genes, Helminth*
Genes, Reporter
Inactivation, Metabolic / genetics
Inactivation, Metabolic / physiology
Longevity / genetics
Longevity / physiology
Mutation
Oxidative Stress
Promoter Regions, Genetic
RNA, Helminth / genetics
RNA, Helminth / metabolism
RNA, Messenger / genetics
RNA, Messenger / metabolism
Signal Transduction
Superoxide Dismutase / genetics*
Superoxide Dismutase / metabolism
Superoxide Dismutase-1
Transcription Factors / metabolism
|
| IF |
3.376
|
| 引用数 |
33
|
|
WOS 分野
|
GERIATRICS & GERONTOLOGY
|
| リソース情報 |
| 線虫 |
NA |
