Reference - Detail
| RRC ID | 46744 |
|---|---|
| Author | Ward JD, Muzzini DM, Petalcorin MI, Martinez-Perez E, Martin JS, Plevani P, Cassata G, Marini F, Boulton SJ. |
| Title | Overlapping mechanisms promote postsynaptic RAD-51 filament disassembly during meiotic double-strand break repair. |
| Journal | Mol Cell |
| Abstract |
Homologous recombination (HR) is essential for repair of meiotic DNA double-strand breaks (DSBs). Although the mechanisms of RAD-51-DNA filament assembly and strand exchange are well characterized, the subsequent steps of HR are less well defined. Here, we describe a synthetic lethal interaction between the C. elegans helicase helq-1 and RAD-51 paralog rfs-1, which results in a block to meiotic DSB repair after strand invasion. Whereas RAD-51-ssDNA filaments assemble at meiotic DSBs with normal kinetics in helq-1, rfs-1 double mutants, persistence of RAD-51 foci and genetic interactions with rtel-1 suggest a failure to disassemble RAD-51 from strand invasion intermediates. Indeed, purified HELQ-1 and RFS-1 independently bind to and promote the disassembly of RAD-51 from double-stranded, but not single-stranded, DNA filaments via distinct mechanisms in vitro. These results indicate that two compensating activities are required to promote postsynaptic RAD-51 filament disassembly, which are collectively essential for completion of meiotic DSB repair. |
| Volume | 37(2) |
| Pages | 259-72 |
| Published | 2010-1-29 |
| DOI | 10.1016/j.molcel.2009.12.026 |
| PII | S1097-2765(09)00957-5 |
| PMID | 20122407 |
| MeSH | Animals Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* DNA Breaks, Double-Stranded* DNA Helicases / genetics DNA Helicases / metabolism DNA Helicases / physiology DNA Repair / genetics DNA Repair / physiology* DNA, Helminth / metabolism DNA, Single-Stranded / metabolism DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism DNA-Binding Proteins / physiology* Meiosis* Mutation Rad51 Recombinase / metabolism* Recombination, Genetic |
| IF | 15.584 |
| Times Cited | 54 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm2134 tm1866 tm1086 tm1268 |