RRC ID 47550
Author Pang H, Qin G, Tan Z, Li Z, Wang Y, Cai Y.
Title Natural populations of lactic acid bacteria associated with silage fermentation as determined by phenotype, 16S ribosomal RNA and recA gene analysis.
Journal Syst Appl Microbiol
Abstract One hundred and fifty-six strains isolated from corn (Zea mays L.), forage paddy rice (Oryza sativa L.), sorghum (Sorghum bicolor L.) and alfalfa (Medicago sativa L.) silages prepared on dairy farms were screened, of which 110 isolates were considered to be lactic acid bacteria (LAB) according to their Gram-positive and catalase-negative characteristics and, mainly, the lactic acid metabolic products. These isolates were divided into eight groups (A-H) based on the following properties: morphological and biochemical characteristics, γ-aminobutyric acid production capacity, and 16S rRNA gene sequences. They were identified as Weissella cibaria (36.4%), Weissella confusa (9.1%), Leuconostoc citreum (5.3%), Leuconostoc lactis (4.9%), Leuconostoc pseudomesenteroides (8.0%), Lactococcus lactis subsp. lactis (4.5%), Lactobacillus paraplantarum (4.5%) and Lactobacillus plantarum (27.3%). W. cibaria and W. confusa were mainly present in corn silages, and L. plantarum was dominant on sorghum and forage paddy rice silages, while L. pseudomesenteroides, L. plantarum and L. paraplantarum were the dominant species in alfalfa silage. The corn, sorghum and forage paddy rice silages were well preserved with lower pH values and ammonia-N concentrations, but had higher lactic acid content, while the alfalfa silage had relatively poor quality with higher pH values and ammonia-N concentrations, and lower lactic acid content. The present study confirmed the diversity of LAB species inhabiting silages. It showed that the differing natural populations of LAB on these silages might influence fermentation quality. These results will enable future research on the relationship between LAB species and silage fermentation quality, and will enhance the screening of appropriate inoculants aimed at improving such quality.
Volume 34(3)
Pages 235-41
Published 2011-5-1
DOI 10.1016/j.syapm.2010.10.003
PII S0723-2020(11)00004-X
PMID 21282025
MeSH Bacterial Proteins / genetics Base Sequence Bile Acids and Salts / metabolism Biodiversity Fermentation Lactic Acid / metabolism Lactobacillales / classification* Lactobacillales / genetics Lactobacillales / isolation & purification Lactobacillales / physiology Medicago sativa / metabolism Medicago sativa / microbiology* Molecular Sequence Data Oryza / metabolism Oryza / microbiology* Phenotype Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S / genetics Rec A Recombinases / genetics Sequence Analysis, DNA Silage / analysis Silage / microbiology* Sorghum / metabolism Sorghum / microbiology* Zea mays / metabolism Zea mays / microbiology* gamma-Aminobutyric Acid / metabolism
IF 3.224
Times Cited 51
General Microbes JCM 5805 JCM 16167 JCM 1180 JCM 1558 JCM 1149 JCM 12533