論文 - 詳細
| RRC ID | 47735 |
|---|---|
| 著者 | Goto Y, Yamagishi Y, Shintomi-Kawamura M, Abe M, Tanno Y, Watanabe Y. |
| タイトル | Pds5 Regulates Sister-Chromatid Cohesion and Chromosome Bi-orientation through a Conserved Protein Interaction Module. |
| ジャーナル | Curr Biol |
| Abstract |
Sister-chromatid cohesion is established by the cohesin complex in S phase and persists until metaphase, when sister chromatids are captured by microtubules emanating from opposite poles [1]. The Aurora-B-containing chromosome passenger complex (CPC) plays a crucial role in achieving chromosome bi-orientation by correcting erroneous microtubule attachment [2]. The centromeric localization of the CPC relies largely on histone H3-T3 phosphorylation (H3-pT3), which is mediated by the mitotic histone kinase Haspin/Hrk1 [3-5]. Hrk1 localization to centromeres depends largely on the cohesin subunit Pds5 in fission yeast [5]; however, it is unknown how Pds5 regulates Hrk1 localization. Here we identify a conserved Hrk1-interacting motif (HIM) in Pds5 and a Pds5-interacting motif (PIM) in Hrk1 in fission yeast. Mutations in either motif result in the displacement of Hrk1 from centromeres. We also show that the mechanism of Pds5-dependent Hrk1 recruitment is conserved in human cells. Notably, the PIM in Haspin/Hrk1 is reminiscent of the YSR motif found in the mammalian cohesin destabilizer Wapl and stabilizer Sororin, both of which bind PDS5 [6-12]. Similarly, and through the same motifs, fission yeast Pds5 binds to Wpl1/Wapl and acetyltransferase Eso1/Eco1, in addition to Hrk1. Thus, we have identified a protein-protein interaction module in Pds5 that serves as a chromatin platform for regulating sister-chromatid cohesion and chromosome bi-orientation. |
| 巻・号 | 27(7) |
| ページ | 1005-1012 |
| 公開日 | 2017-4-3 |
| DOI | 10.1016/j.cub.2017.02.066 |
| PII | S0960-9822(17)30268-3 |
| PMID | 28343969 |
| MeSH | Amino Acid Sequence Cell Cycle Proteins / chemistry Cell Cycle Proteins / genetics* Cell Cycle Proteins / metabolism Chromosome Segregation DNA-Binding Proteins / chemistry DNA-Binding Proteins / genetics* DNA-Binding Proteins / metabolism Humans Potassium Channels, Inwardly Rectifying / chemistry Potassium Channels, Inwardly Rectifying / genetics* Potassium Channels, Inwardly Rectifying / metabolism Protein Serine-Threonine Kinases / chemistry Protein Serine-Threonine Kinases / genetics* Protein Serine-Threonine Kinases / metabolism Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / chemistry Schizosaccharomyces pombe Proteins / genetics* Schizosaccharomyces pombe Proteins / metabolism Sequence Alignment Transcription Factors / chemistry Transcription Factors / genetics* Transcription Factors / metabolism |
| IF | 9.601 |
| 引用数 | 21 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FY24375(PL874) FY25107(PJ283) FY24020(JY450) FY25118(PJ330) |