RRC ID 4820
Author Komatsu K, Nishikawa Y, Ohtsuka T, Taji T, Quatrano RS, Tanaka S, Sakata Y.
Title Functional analyses of the ABI1-related protein phosphatase type 2C reveal evolutionarily conserved regulation of abscisic acid signaling between Arabidopsis and the moss Physcomitrella patens.
Journal Plant Mol. Biol.
Abstract We employed a comparative genomic approach to understand protein phosphatase 2C (PP2C)-mediated abscisic acid (ABA) signaling in the moss Physcomitrella patens. Ectopic expression of Arabidopsis (Arabidopsis thaliana) abi1-1, a dominant mutant allele of ABI1 encoding a PP2C involved in the negative regulation of ABA signaling, caused ABA insensitivity of P. patens both in gene expression of late embryogenesis abundant (LEA) genes and in ABA-induced protonemal growth inhibition. The transgenic abi1-1 plants showed decreased ABA-induced freezing tolerance, and decreased tolerance to osmotic stress. Analyses of the P. patens genome revealed that only two (PpABI1A and PpABI1B) PP2C genes were related to ABI1. In the ppabi1a null mutants, ABA-induced expression of LEA genes was elevated, and protonemal growth was inhibited with lower ABA concentration compared to the wild type. Moreover, ABA-induced freezing tolerance of the ppabi1a mutants was markedly enhanced. We provide the genetic evidence that PP2C-mediated ABA signaling is evolutionarily conserved between Arabidopsis and P. patens.
Volume 70(3)
Pages 327-40
Published 2009-6
DOI 10.1007/s11103-009-9476-z
PMID 19266168
MeSH Abscisic Acid / metabolism* Arabidopsis / enzymology* Arabidopsis / genetics Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Base Sequence Bryopsida / enzymology* Bryopsida / genetics Cloning, Molecular Evolution, Molecular Gene Expression Regulation, Plant Genome, Plant Molecular Sequence Data Mutation Phosphoprotein Phosphatases / genetics Phosphoprotein Phosphatases / metabolism* Phylogeny Plants, Genetically Modified / enzymology Plants, Genetically Modified / genetics Protein Phosphatase 2C RNA, Plant / genetics Signal Transduction* Stress, Physiological
IF 3.928
Times Cited 36
WOS Category PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Arabidopsis / Cultured plant cells, genes pdp31463