論文 - 詳細
| RRC ID | 49577 |
|---|---|
| 著者 | Ren B, Tan HL, Nguyen TTT, Sayed AMM, Li Y, Mok YK, Yang H, Chen ES. |
| タイトル | Regulation of transcriptional silencing and chromodomain protein localization at centromeric heterochromatin by histone H3 tyrosine 41 phosphorylation in fission yeast. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
Heterochromatin silencing is critical for genomic integrity and cell survival. It is orchestrated by chromodomain (CD)-containing proteins that bind to methylated histone H3 lysine 9 (H3K9me), a hallmark of heterochromatin. Here, we show that phosphorylation of tyrosine 41 (H3Y41p)-a novel histone H3 modification-participates in the regulation of heterochromatin in fission yeast. We show that a loss-of-function mutant of H3Y41 can suppress heterochromatin de-silencing in the centromere and subtelomere repeat regions, suggesting a de-silencing role for H3Y41p on heterochromatin. Furthermore, we show both in vitro and in vivo that H3Y41p differentially regulates two CD-containing proteins without the change in the level of H3K9 methylation: it promotes the binding of Chp1 to histone H3 and the exclusion of Swi6. H3Y41p is preferentially enriched on centromeric heterochromatin during M- to early S phase, which coincides with the localization switch of Swi6/Chp1. The loss-of-function H3Y41 mutant could suppress the hypersensitivity of the RNAi mutants towards hydroxyurea (HU), which arrests replication in S phase. Overall, we describe H3Y41p as a novel histone modification that differentially regulates heterochromatin silencing in fission yeast via the binding of CD-containing proteins. |
| 巻・号 | 46(1) |
| ページ | 189-202 |
| 公開日 | 2018-1-9 |
| DOI | 10.1093/nar/gkx1010 |
| PII | 4612962 |
| PMID | 29136238 |
| PMC | PMC5758876 |
| MeSH | Cell Cycle Checkpoints / genetics Centromere / genetics* Centromere / metabolism Heterochromatin / genetics* Heterochromatin / metabolism Histones / genetics* Histones / metabolism Loss of Function Mutation Methylation Phosphorylation RNA Interference* S Phase / genetics Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / genetics* Schizosaccharomyces pombe Proteins / metabolism Tyrosine / genetics Tyrosine / metabolism |
| IF | 11.502 |
| 引用数 | 2 |
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| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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