RRC ID 50322
Author Brown SD, Jarosinska OD, Lorenz A.
Title Genetic interactions between the chromosome axis-associated protein Hop1 and homologous recombination determinants in Schizosaccharomyces pombe.
Journal Curr Genet
Abstract Hop1 is a component of the meiosis-specific chromosome axis and belongs to the evolutionarily conserved family of HORMA domain proteins. Hop1 and its orthologs in higher eukaryotes are a major factor in promoting double-strand DNA break formation and inter-homolog recombination. In budding yeast and mammals, they are also involved in a meiotic checkpoint kinase cascade monitoring the completion of double-strand DNA break repair. We used the fission yeast, Schizosaccharomyces pombe, which lacks a canonical synaptonemal complex to test whether Hop1 has a role beyond supporting the generation of double-strand DNA breaks and facilitating inter-homolog recombination events. We determined how mutants of homologous recombination factors genetically interact with hop1, studied the role(s) of the HORMA domain of Hop1, and characterized a bio-informatically predicted interactor of Hop1, Aho1 (SPAC688.03c). Our observations indicate that in fission yeast, Hop1 does require its HORMA domain to support wild-type levels of meiotic recombination and localization to meiotic chromatin. Furthermore, we show that hop1∆ only weakly interacts genetically with mutants of homologous recombination factors, and in fission yeast likely has no major role beyond break formation and promoting inter-homolog events. We speculate that after the evolutionary loss of the synaptonemal complex, Hop1 likely has become less important for modulating recombination outcome during meiosis in fission yeast, and that this led to a concurrent rewiring of genetic pathways controlling meiotic recombination.
Volume 64(5)
Pages 1089-1104
Published 2018-10-1
DOI 10.1007/s00294-018-0827-7
PII 10.1007/s00294-018-0827-7
PMID 29550859
PMC PMC6153652
MeSH Amino Acid Sequence Chromosomes, Fungal* DNA Breaks, Double-Stranded DNA Helicases / genetics DNA-Binding Proteins / chemistry DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Endonucleases / genetics Evolution, Molecular Genes, Fungal Homologous Recombination* Meiosis / genetics Protein Binding Recombinases / genetics Schizosaccharomyces / genetics* Schizosaccharomyces pombe Proteins / chemistry Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Sequence Homology, Amino Acid Synaptonemal Complex / genetics
IF 3.464
Times Cited 4
Yeast FY18530