Reference - Detail
| RRC ID | 51511 |
|---|---|
| Author | Liu H, Wang S, Hang W, Gao J, Zhang W, Cheng Z, Yang C, He J, Zhou J, Chen J, Shi A. |
| Title | LET-413/Erbin acts as a RAB-5 effector to promote RAB-10 activation during endocytic recycling. |
| Journal | J Cell Biol |
| Abstract |
RAB-10/Rab10 is a master regulator of endocytic recycling in epithelial cells. To better understand the regulation of RAB-10 activity, we sought to identify RAB-10(GDP)-interacting proteins. One novel RAB-10(GDP)-binding partner that we identified, LET-413, is the Caenorhabditis elegans homologue of Scrib/Erbin. Here, we focus on the mechanistic role of LET-413 in the regulation of RAB-10 within the C. elegans intestine. We show that LET-413 is a RAB-5 effector and colocalizes with RAB-10 on endosomes, and the overlap of LET-413 with RAB-10 is RAB-5 dependent. Notably, LET-413 enhances the interaction of DENN-4 with RAB-10(GDP) and promotes DENN-4 guanine nucleotide exchange factor activity toward RAB-10. Loss of LET-413 leads to cytosolic dispersion of the RAB-10 effectors TBC-2 and CNT-1. Finally, we demonstrate that the loss of RAB-10 or LET-413 results in abnormal overextensions of lateral membrane. Hence, our studies indicate that LET-413 is required for DENN-4-mediated RAB-10 activation, and the LET-413-assisted RAB-5 to RAB-10 cascade contributes to the integrity of C. elegans intestinal epithelia. |
| Volume | 217(1) |
| Pages | 299-314 |
| Published | 2018-1-2 |
| DOI | 10.1083/jcb.201705136 |
| PII | jcb.201705136 |
| PMID | 29079669 |
| PMC | PMC5748983 |
| MeSH | Animals Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Line, Tumor Cell Polarity / physiology Endocytosis / physiology Enzyme Activation Epithelial Cells / metabolism GTPase-Activating Proteins / metabolism Guanine Nucleotide Exchange Factors / metabolism Guanylate Kinases / genetics HeLa Cells Humans Intestinal Mucosa / physiology* Protein Transport / physiology Vesicular Transport Proteins / metabolism* rab GTP-Binding Proteins / metabolism* |
| IF | 8.811 |
| Times Cited | 11 |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 23 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm1447 tm2523 tm2616 |