| RRC ID |
51829
|
| 著者 |
Kitakaze T, Sakamoto T, Kitano T, Inoue N, Sugihara F, Harada N, Yamaji R.
|
| タイトル |
The collagen derived dipeptide hydroxyprolyl-glycine promotes C2C12 myoblast differentiation and myotube hypertrophy.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
The majority of studies on possible roles for collagen hydrolysates in human health have focused on their effects on bone and skin. Hydroxyprolyl-glycine (Hyp-Gly) was recently identified as a novel collagen hydrolysate-derived dipeptide in human blood. However, any possible health benefits of Hyp-Gly remain unclear. Here, we report the effects of Hyp-Gly on differentiation and hypertrophy of murine skeletal muscle C2C12 cells. Hyp-Gly increased the fusion index, the myotube size, and the expression of the myotube-specific myosin heavy chain (MyHC) and tropomyosin structural proteins. Hyp-Gly increased the phosphorylation of Akt, mTOR, and p70S6K in myoblasts, whereas the phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 inhibited their phosphorylation by Hyp-Gly. LY294002 and the mammalian target of rapamycin (mTOR) inhibitor rapamycin repressed the enhancing effects of Hyp-Gly on MyHC and tropomyosin expression. The peptide/histidine transporter 1 (PHT1) was highly expressed in both myoblasts and myotubes, and co-administration of histidine inhibited Hyp-Gly-induced phosphorylation of p70S6K in myoblasts and myotubes. These results indicate that Hyp-Gly can induce myogenic differentiation and myotube hypertrophy and suggest that Hyp-Gly promotes myogenic differentiation by activating the PI3K/Akt/mTOR signaling pathway, perhaps depending on PHT1 for entry into cells.
|
| 巻・号 |
478(3)
|
| ページ |
1292-7
|
| 公開日 |
2016-9-23
|
| DOI |
10.1016/j.bbrc.2016.08.114
|
| PII |
S0006-291X(16)31378-X
|
| PMID |
27553280
|
| MeSH |
Animals
Cell Differentiation / drug effects*
Cell Size / drug effects
Collagen / pharmacology*
Dipeptides / pharmacology*
Histidine / pharmacology
Hypertrophy
Male
Membrane Transport Proteins / metabolism
Mice
Muscle Fibers, Skeletal / drug effects
Muscle Fibers, Skeletal / metabolism
Muscle Fibers, Skeletal / pathology*
Myoblasts / cytology*
Myoblasts / drug effects
Myoblasts / metabolism
Myosin Heavy Chains / metabolism
Phosphatidylinositol 3-Kinases / metabolism
Phosphorylation
Proto-Oncogene Proteins c-akt / metabolism
Ribosomal Protein S6 Kinases, 70-kDa / metabolism
Signal Transduction / drug effects
TOR Serine-Threonine Kinases / metabolism
Tropomyosin / metabolism
|
| IF |
2.985
|
| 引用数 |
15
|
| リソース情報 |
| ヒト・動物細胞 |
C2C12(RCB0987) |
