RRC ID 52001
Author Tanaka S, Maekawa A, Matsubara L, Imanishi A, Yano M, Roeder RG, Hasegawa N, Asano S, Ito M.
Title Periostin supports hematopoietic progenitor cells and niche-dependent myeloblastoma cells in vitro.
Journal Biochem Biophys Res Commun
Abstract The expression of extracellular matrix protein periostin (POSTN) was attenuated in Med1(-/-) mouse embryonic fibroblasts (MEFs), which exhibited a decreased capability to support hematopoietic progenitor cells (HPCs) in vitro. When bone marrow (BM) cells were cocultured with mitomycin C-treated Med1(+/+) MEFs, or OP-9 or MS-5 BM stromal cells, in the presence of anti-POSTN antibody, the growth of BM cells and number of long-term culture-initiating cells (LTC-ICs) were attenuated. When BM cells were cocultured with Med1(-/-) MEFs in the presence of recombinant POSTN, the growth of BM cells and the number of LTC-ICs were restored. Moreover, antibody-mediated blockage of stromal cells-derived POSTN markedly reduced the growth and cobblestone formation, a leukemic stem cell feature, of stromal cell-dependent MB-1 myeloblastoma cells. POSTN was expressed both in BM cells and variably in different BM stromal cells. Expression in the latter cells was increased by physical interaction with hematopoietic cells. The receptor for POSTN, integrin αvβ3, was expressed abundantly in BM stromal cells. The addition of recombinant POSTN to BM stromal cells induced intracellular signaling downstream of integrin αvβ3. These results suggest that stromal cell POSTN supports both normal HPCs and leukemia-initiating cells in vitro, at least in part, indirectly by acting on stromal cells in an autocrine or paracrine manner.
Volume 478(4)
Pages 1706-12
Published 2016-9-30
DOI 10.1016/j.bbrc.2016.09.008
PII S0006-291X(16)31457-7
PMID 27596966
PMC PMC5808943
MeSH Animals Blotting, Western Cell Adhesion Molecules / genetics* Cell Adhesion Molecules / metabolism Cell Adhesion Molecules / pharmacology Cell Line Cell Line, Tumor Cell Proliferation / genetics Cells, Cultured Coculture Techniques Embryo, Mammalian / cytology Fibroblasts / cytology Fibroblasts / metabolism Gene Expression* HEK293 Cells Hematopoietic Stem Cells / metabolism* Humans Integrin alphaVbeta3 / genetics Integrin alphaVbeta3 / metabolism Mediator Complex Subunit 1 / genetics Mediator Complex Subunit 1 / metabolism Mesenchymal Stem Cells / metabolism Mice, Inbred C57BL Mice, Knockout Reverse Transcriptase Polymerase Chain Reaction Sarcoma, Myeloid / genetics Sarcoma, Myeloid / metabolism Sarcoma, Myeloid / pathology Signal Transduction / drug effects Signal Transduction / genetics Stem Cell Niche*
IF 2.705
Times Cited 4
Human and Animal Cells OP9(RCB1124)