| RRC ID |
53246
|
| Author |
Neal SJ, Park J, DiTirro D, Yoon J, Shibuya M, Choi W, Schroeder FC, Butcher RA, Kim K, Sengupta P.
|
| Title |
A Forward Genetic Screen for Molecules Involved in Pheromone-Induced Dauer Formation in Caenorhabditis elegans.
|
| Journal |
G3 (Bethesda)
|
| Abstract |
Animals must constantly assess their surroundings and integrate sensory cues to make appropriate behavioral and developmental decisions. Pheromones produced by conspecific individuals provide critical information regarding environmental conditions. Ascaroside pheromone concentration and composition are instructive in the decision of Caenorhabditis elegans to either develop into a reproductive adult or enter into the stress-resistant alternate dauer developmental stage. Pheromones are sensed by a small set of sensory neurons, and integrated with additional environmental cues, to regulate neuroendocrine signaling and dauer formation. To identify molecules required for pheromone-induced dauer formation, we performed an unbiased forward genetic screen and identified phd (pheromone response-defective dauer) mutants. Here, we describe new roles in dauer formation for previously identified neuronal molecules such as the WD40 domain protein QUI-1 and MACO-1 Macoilin, report new roles for nociceptive neurons in modulating pheromone-induced dauer formation, and identify tau tubulin kinases as new genes involved in dauer formation. Thus, phd mutants define loci required for the detection, transmission, or integration of pheromone signals in the regulation of dauer formation.
|
| Volume |
6(5)
|
| Pages |
1475-87
|
| Published |
2016-5-3
|
| DOI |
10.1534/g3.115.026450
|
| PII |
g3.115.026450
|
| PMID |
26976437
|
| PMC |
PMC4856098
|
| MeSH |
Animals
Caenorhabditis elegans / drug effects*
Caenorhabditis elegans / genetics*
Caenorhabditis elegans / growth & development
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism
Chromosome Mapping
Genetic Association Studies*
Genetic Complementation Test
Genetic Linkage
Genetic Testing*
Genome, Helminth
Genomics / methods
High-Throughput Nucleotide Sequencing
Mutation
Pheromones / pharmacology*
Signal Transduction / drug effects
Stress, Physiological / drug effects*
Stress, Physiological / genetics*
|
| IF |
2.781
|
| Times Cited |
8
|
| Resource |
| C.elegans |
tm4006
tm4134
tm3841
tm4852
tm1946
tm2943 |
