論文 - 詳細
| RRC ID | 53290 |
|---|---|
| 著者 | Lin JLJ, Nakagawa A, Skeen-Gaar R, Yang WZ, Zhao P, Zhang Z, Ge X, Mitani S, Xue D, Yuan HS. |
| タイトル | Oxidative Stress Impairs Cell Death by Repressing the Nuclease Activity of Mitochondrial Endonuclease G. |
| ジャーナル | Cell Rep |
| Abstract |
Endonuclease G (EndoG) is a mitochondrial protein that is released from mitochondria and relocated into the nucleus to promote chromosomal DNA fragmentation during apoptosis. Here, we show that oxidative stress causes cell-death defects in C. elegans through an EndoG-mediated cell-death pathway. In response to high reactive oxygen species (ROS) levels, homodimeric CPS-6-the C. elegans homolog of EndoG-is dissociated into monomers with diminished nuclease activity. Conversely, the nuclease activity of CPS-6 is enhanced, and its dimeric structure is stabilized by its interaction with the worm AIF homolog, WAH-1, which shifts to disulfide cross-linked dimers under high ROS levels. CPS-6 thus acts as a ROS sensor to regulate the life and death of cells. Modulation of the EndoG dimer conformation could present an avenue for prevention and treatment of diseases resulting from oxidative stress. |
| 巻・号 | 16(2) |
| ページ | 279-287 |
| 公開日 | 2016-7-12 |
| DOI | 10.1016/j.celrep.2016.05.090 |
| PII | S2211-1247(16)30703-3 |
| PMID | 27346342 |
| PMC | PMC5483177 |
| MeSH | Animals Apoptosis* Caenorhabditis elegans / cytology Caenorhabditis elegans / enzymology* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / metabolism* Crystallography, X-Ray Down-Regulation Endodeoxyribonucleases / chemistry Endodeoxyribonucleases / metabolism* Enzyme Stability Mitochondrial Proteins / chemistry Mitochondrial Proteins / metabolism* Models, Molecular Oxidation-Reduction Oxidative Stress* Protein Interaction Domains and Motifs Protein Multimerization Protein Structure, Quaternary |
| IF | 8.109 |
| 引用数 | 11 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1159 |