RRC ID 53296
Author Guan L, Ma X, Zhang J, Liu JJ, Wang Y, Ding M.
Title The Calponin Family Member CHDP-1 Interacts with Rac/CED-10 to Promote Cell Protrusions.
Journal PLoS Genet
Abstract Eukaryotic cells extend a variety of surface protrusions to direct cell motility. Formation of protrusions is mediated by coordinated actions between the plasma membrane and the underlying actin cytoskeleton. Here, we found that the single calponin homology (CH) domain-containing protein CHDP-1 induces the formation of cell protrusions in C. elegans. CHDP-1 is anchored to the cortex through its amphipathic helix. CHDP-1 associates through its CH domain with the small GTPase Rac1/CED-10, which is a key regulator of the actin cytoskeleton. CHDP-1 preferentially binds to the GTP-bound active form of the CED-10 protein and preserves the membrane localization of GTP-CED-10. Hence, by coupling membrane expansion to Rac1-mediated actin dynamics, CHDP-1 promotes the formation of cellular protrusions in vivo.
Volume 12(7)
Pages e1006163
Published 2016-7
DOI 10.1371/journal.pgen.1006163
PMID 27415421
PMC PMC4944944
MeSH Actin Cytoskeleton / metabolism Actins / metabolism Alleles Animals Animals, Genetically Modified Caenorhabditis elegans Caenorhabditis elegans Proteins / metabolism* Calcium-Binding Proteins / metabolism* Cell Membrane / metabolism Cell Movement Cell Surface Extensions / metabolism* Gene Expression Regulation* Microfilament Proteins / metabolism* Models, Genetic Monomeric GTP-Binding Proteins / metabolism Neurons / metabolism Phenotype Protein Binding Sequence Analysis, DNA Transgenes rac GTP-Binding Proteins / metabolism*
IF 5.224
Times Cited 3
C.elegans tm4947