論文 - 詳細
| RRC ID | 53440 |
|---|---|
| 著者 | Wu F, Watanabe Y, Guo XY, Qi X, Wang P, Zhao HY, Wang Z, Fujioka Y, Zhang H, Ren JQ, Fang TC, Shen YX, Feng W, Hu JJ, Noda NN, Zhang H. |
| タイトル | Structural Basis of the Differential Function of the Two C. elegans Atg8 Homologs, LGG-1 and LGG-2, in Autophagy. |
| ジャーナル | Mol Cell |
| Abstract |
Multicellular organisms have multiple homologs of the yeast ATG8 gene, but the differential roles of these homologs in autophagy during development remain largely unknown. Here we investigated structure/function relationships in the two C. elegans Atg8 homologs, LGG-1 and LGG-2. lgg-1 is essential for degradation of protein aggregates, while lgg-2 has cargo-specific and developmental-stage-specific roles in aggregate degradation. Crystallography revealed that the N-terminal tails of LGG-1 and LGG-2 adopt the closed and open form, respectively. LGG-1 and LGG-2 interact differentially with autophagy substrates and Atg proteins, many of which carry a LIR motif. LGG-1 and LGG-2 have structurally distinct substrate binding pockets that prefer different residues in the interacting LIR motif, thus influencing binding specificity. Lipidated LGG-1 and LGG-2 possess distinct membrane tethering and fusion activities, which may result from the N-terminal differences. Our study reveals the differential function of two ATG8 homologs in autophagy during C. elegans development. |
| 巻・号 | 60(6) |
| ページ | 914-29 |
| 公開日 | 2015-12-17 |
| DOI | 10.1016/j.molcel.2015.11.019 |
| PII | S1097-2765(15)00911-9 |
| PMID | 26687600 |
| MeSH | Animals Autophagy* Autophagy-Related Protein 8 Family Binding Sites Caenorhabditis elegans / chemistry Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / chemistry* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Crystallography, X-Ray Microtubule-Associated Proteins / chemistry* Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism Models, Molecular Mutation Protein Conformation Protein Serine-Threonine Kinases / metabolism Saccharomyces cerevisiae / chemistry Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae Proteins / chemistry Saccharomyces cerevisiae Proteins / genetics |
| IF | 15.584 |
| 引用数 | 43 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm6474 tm2508 tm3425 |