論文 - 詳細
| RRC ID | 53454 |
|---|---|
| 著者 | Liu K, Jian Y, Sun X, Yang C, Gao Z, Zhang Z, Liu X, Li Y, Xu J, Jing Y, Mitani S, He S, Yang C. |
| タイトル | Negative regulation of phosphatidylinositol 3-phosphate levels in early-to-late endosome conversion. |
| ジャーナル | J Cell Biol |
| Abstract |
Phosphatidylinositol 3-phosphate (PtdIns3P) plays a central role in endosome fusion, recycling, sorting, and early-to-late endosome conversion, but the mechanisms that determine how the correct endosomal PtdIns3P level is achieved remain largely elusive. Here we identify two new factors, SORF-1 and SORF-2, as essential PtdIns3P regulators in Caenorhabditis elegans. Loss of sorf-1 or sorf-2 leads to greatly elevated endosomal PtdIns3P, which drives excessive fusion of early endosomes. sorf-1 and sorf-2 function coordinately with Rab switching genes to inhibit synthesis of PtdIns3P, allowing its turnover for endosome conversion. SORF-1 and SORF-2 act in a complex with BEC-1/Beclin1, and their loss causes elevated activity of the phosphatidylinositol 3-kinase (PI3K) complex. In mammalian cells, inactivation of WDR91 and WDR81, the homologs of SORF-1 and SORF-2, induces Beclin1-dependent enlargement of PtdIns3P-enriched endosomes and defective degradation of epidermal growth factor receptor. WDR91 and WDR81 interact with Beclin1 and inhibit PI3K complex activity. These findings reveal a conserved mechanism that controls appropriate PtdIns3P levels in early-to-late endosome conversion. |
| 巻・号 | 212(2) |
| ページ | 181-98 |
| 公開日 | 2016-1-18 |
| DOI | 10.1083/jcb.201506081 |
| PII | jcb.201506081 |
| PMID | 26783301 |
| PMC | PMC4738380 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism* Carrier Proteins / metabolism* Endosomes / metabolism* Membrane Fusion Mutation Nerve Tissue Proteins / metabolism Phosphatidylinositol Phosphates / metabolism* Vesicular Transport Proteins / metabolism rab GTP-Binding Proteins / metabolism |
| IF | 8.811 |
| 引用数 | 21 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3855 tm5210 tm5202 tm1125 tm2241 tm4475 tm327 tm2253 tm3171 |