論文 - 詳細
| RRC ID | 5684 |
|---|---|
| 著者 | Shi A, Sun L, Banerjee R, Tobin M, Zhang Y, Grant BD. |
| タイトル | Regulation of endosomal clathrin and retromer-mediated endosome to Golgi retrograde transport by the J-domain protein RME-8. |
| ジャーナル | EMBO J |
| Abstract |
After endocytosis, most cargo enters the pleiomorphic early endosomes in which sorting occurs. As endosomes mature, transmembrane cargo can be sequestered into inwardly budding vesicles for degradation, or can exit the endosome in membrane tubules for recycling to the plasma membrane, the recycling endosome, or the Golgi apparatus. Endosome to Golgi transport requires the retromer complex. Without retromer, recycling cargo such as the MIG-14/Wntless protein aberrantly enters the degradative pathway and is depleted from the Golgi. Endosome-associated clathrin also affects the recycling of retrograde cargo and has been shown to function in the formation of endosomal subdomains. Here, we find that the Caemorhabditis elegans endosomal J-domain protein RME-8 associates with the retromer component SNX-1. Loss of SNX-1, RME-8, or the clathrin chaperone Hsc70/HSP-1 leads to over-accumulation of endosomal clathrin, reduced clathrin dynamics, and missorting of MIG-14 to the lysosome. Our results indicate a mechanism, whereby retromer can regulate endosomal clathrin dynamics through RME-8 and Hsc70, promoting the sorting of recycling cargo into the retrograde pathway. |
| 巻・号 | 28(21) |
| ページ | 3290-302 |
| 公開日 | 2009-11-4 |
| DOI | 10.1038/emboj.2009.272 |
| PII | emboj2009272 |
| PMID | 19763082 |
| PMC | PMC2776105 |
| MeSH | Animals Caenorhabditis elegans / cytology* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / analysis Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Carrier Proteins / analysis Carrier Proteins / metabolism Cell Line Cell Polarity Clathrin / metabolism* Endosomes / metabolism* Golgi Apparatus / genetics HSC70 Heat-Shock Proteins / metabolism Intracellular Signaling Peptides and Proteins Lysosomes / metabolism Molecular Chaperones Mutation Neurons / cytology Sorting Nexins Vesicular Transport Proteins / analysis Vesicular Transport Proteins / genetics Vesicular Transport Proteins / metabolism* |
| IF | 9.889 |
| 引用数 | 94 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | snx-1(tm0847) tm847 tm857 |