RRC ID 6075
著者 Takeo S, Tsuda M, Akahori S, Matsuo T, Aigaki T.
タイトル The calcineurin regulator sra plays an essential role in female meiosis in Drosophila.
ジャーナル Curr Biol
Abstract Modulatory calcineurin-interacting proteins (MCIPs)--also termed regulators of calcineurin (RCNs), calcipressins, or DSCR1 (Down's syndrome critical region 1)--are highly conserved regulators of calcineurin, a Ca(2+)/calmodulin-dependent protein phosphatase . Although overexpression experiments in several organisms have revealed that MCIPs inhibit calcineurin activity , their in vivo functions remain unclear. Here, we show that the Drosophila MCIP sarah (sra) is essential for meiotic progression in oocytes. Eggs from sra null mothers are arrested at anaphase of meiosis I. This phenotype was due to loss of function of sra specifically in the female germline. Sra is physically associated with the catalytic subunit of calcineurin, and its overexpression suppresses the phenotypes caused by constitutively activated calcineurin, such as rough eye or loss of wing veins. Hyperactivation of calcineurin signaling in the germline cells resulted in a meiotic-arrest phenotype, which can also be suppressed by overexpression of Sra. All these results support the hypothesis that Sra regulates female meiosis by controlling calcineurin activity in the germline. To our knowledge, this is the first unambiguous demonstration that the regulation of calcineurin signaling by MCIPs plays a critical role in a defined biological process.
巻・号 16(14)
ページ 1435-40
公開日 2006-7-25
DOI 10.1016/j.cub.2006.05.058
PII S0960-9822(06)01677-0
PMID 16860743
MeSH Anaphase / genetics Animals Calcineurin / genetics Calcineurin / metabolism* Calcium-Binding Proteins Drosophila / anatomy & histology Drosophila / cytology Drosophila / metabolism* Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila Proteins / physiology* Female Fertility / genetics Gene Expression Regulation Intracellular Signaling Peptides and Proteins / genetics Intracellular Signaling Peptides and Proteins / metabolism Intracellular Signaling Peptides and Proteins / physiology* Meiosis* / genetics Movement / physiology Mutation Ovary / cytology Ovary / metabolism Ovum / cytology* Ovum / metabolism Phenotype Protein Subunits / metabolism Signal Transduction
IF 9.601
引用数 51
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ショウジョウバエ DGRC#109861 DGRC#109863