論文 - 詳細
| RRC ID | 63142 |
|---|---|
| 著者 | Miyata S, Nakamura T, Kitanaka S. |
| タイトル | 3EZ, 20Ac-ingenol-induced Apoptosis in Chemoresistant Cancers With Cyclin D1 Accumulation. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND/AIM:The topoisomerase 1 catalytic inhibitor 3EZ, 20Ac-ingenol specifically induces apoptosis through the activation of ATR and the up-regulation of PTEN by enhancing the DNA damage response (DDR) in human B lymphoma (BALL-1) cells. The accumulation of cyclin D1 in cancer is known to be related to chemoresistance to DNA damage agents and nuclei of BALL-1 cells exhibit high levels of cyclin D1. However, 3EZ, 20Ac-ingenol effectively induced apoptosis of BALL-1 cells. MATERIALS AND METHODS:Cell growth, protein levels, and apoptosis were determined by an MTT assay, immunoblotting and DNA fragmentation assay, respectively. RESULTS:3EZ, 20Ac-ingenol strongly induced inhibition of cell proliferation and apoptosis in Jeko-1 and Panc-1 cell lines through the activation of tumor suppressor proteins and caspase 3. CONCLUSION:3EZ, 20Ac-ingenol-induced apoptosis might occur in cells with cyclin D1 accumulation through enhancing DDR, regardless of the cancer cell type. |
| 巻・号 | 40(11) |
| ページ | 6237-6246 |
| 公開日 | 2020-11-1 |
| DOI | 10.21873/anticanres.14644 |
| PII | 40/11/6237 |
| PMID | 33109561 |
| MeSH | Apoptosis / drug effects* Ataxia Telangiectasia Mutated Proteins / metabolism Caspase 3 / metabolism Cell Line, Tumor Cell Proliferation / drug effects Cyclin A / metabolism Cyclin D1 / metabolism* Cyclin-Dependent Kinase Inhibitor p21 / metabolism Diterpenes / pharmacology* Drug Resistance, Neoplasm / drug effects* Enzyme Activation / drug effects Glycogen Synthase Kinase 3 beta / metabolism Histones / metabolism Humans Irinotecan / pharmacology PTEN Phosphohydrolase / metabolism Phosphorylation / drug effects Proto-Oncogene Proteins c-akt / metabolism Signal Transduction / drug effects Tumor Suppressor Protein p53 / metabolism |
| IF | 1.994 |
| リソース情報 | |
| ヒト・動物細胞 | PANC-1(RCB2095) |