論文 - 詳細
| RRC ID | 6405 |
|---|---|
| 著者 | Saheki Y, Bargmann CI. |
| タイトル | Presynaptic CaV2 calcium channel traffic requires CALF-1 and the alpha(2)delta subunit UNC-36. |
| ジャーナル | Nat Neurosci |
| Abstract |
Presynaptic voltage-gated calcium channels provide calcium for synaptic vesicle exocytosis. We show here that a green fluorescent protein-tagged alpha(1) subunit of the Caenorhabditis elegans CaV2 channel, UNC-2, is localized to presynaptic active zones of sensory and motor neurons. Synaptic localization of CaV2 requires the alpha(2)delta subunit UNC-36 and CALF-1 (Calcium Channel Localization Factor-1), a neuronal transmembrane protein that localizes to the endoplasmic reticulum. In calf-1 mutants, UNC-2 is retained in the endoplasmic reticulum, but other active-zone components and synaptic vesicles are delivered to synapses. Acute induction of calf-1 mobilizes preexisting UNC-2 for delivery to synapses, consistent with a direct trafficking role. The alpha(2)delta subunit UNC-36 is likewise required for exit of UNC-2 from endoplasmic reticulum but has additional functions. Genetic and cell biological interactions suggest that CALF-1 couples intracellular traffic to functional maturation of CaV2 presynaptic calcium channels. |
| 巻・号 | 12(10) |
| ページ | 1257-65 |
| 公開日 | 2009-10-1 |
| DOI | 10.1038/nn.2383 |
| PII | nn.2383 |
| PMID | 19718034 |
| PMC | PMC2805665 |
| MeSH | Animals Animals, Genetically Modified Behavior, Animal Caenorhabditis elegans Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* Calcium / metabolism Calcium Channels / metabolism* Chi-Square Distribution Chromosome Mapping / methods Endoplasmic Reticulum / metabolism Epithelial Cells / metabolism Epithelial Cells / ultrastructure Exocytosis Gene Expression Regulation / genetics Gene Expression Regulation / physiology* Green Fluorescent Proteins / genetics Intestines / cytology Membrane Proteins / genetics Membrane Proteins / metabolism Membrane Proteins / physiology* Mutation Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism Neurons / cytology Neurons / metabolism Neurons / ultrastructure Presynaptic Terminals / metabolism* Protein Subunits / metabolism Protein Transport / genetics Protein Transport / physiology Swimming / physiology Synapses / physiology Vacuolar Proton-Translocating ATPases / physiology* |
| IF | 20.071 |
| 引用数 | 56 |
| WOS 分野 | NEUROSCIENCES |
| リソース情報 | |
| 線虫 | tm1233 |