論文 - 詳細
| RRC ID | 64366 |
|---|---|
| 著者 | Iwahashi N, Ikezaki M, Nishikawa T, Namba N, Ohgita T, Saito H, Ihara Y, Shimanouchi T, Ino K, Uchimura K, Nishitsuji K. |
| タイトル | Sulfated glycosaminoglycans mediate prion-like behavior of p53 aggregates. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Sulfated glycosaminoglycans (GAGs) such as heparan sulfate (HS) are heteropolysaccharides implicated in the pathology of protein aggregation diseases including localized and systemic forms of amyloidosis. Among subdomains of sulfated GAGs, highly sulfated domains of HS, called HS S-domains, have been highlighted as being critical for HS function in amyloidoses. Recent studies suggest that the tumor suppressor p53 aggregates to form amyloid fibrils and propagates in a prion-like manner; however, molecules and mechanisms that are involved in the prion-like behavior of p53 aggregates have not been addressed. Here, we identified sulfated GAGs as molecules that mediate prion-like behavior of p53 aggregates. Sulfated GAGs at the cell surface were required for cellular uptake of recombinant and cancer cell-derived p53 aggregates and extracellular release of p53 from cancer cells. We further showed that HS S-domains accumulated within p53 deposits in human ovarian cancer tissues, and enzymatic remodeling of HS S-domains by Sulf-2 extracellular sulfatase down-regulated cellular uptake of p53 aggregates. Finally, sulfated GAG-dependent cellular uptake of p53 aggregates was critical for subsequent extracellular release of the aggregates and gain of oncogenic function in recipient cells. Our work provides a mechanism of prion-like behavior of p53 aggregates and will shed light on sulfated GAGs as a common mediator of prions. |
| 巻・号 | 117(52) |
| ページ | 33225-33234 |
| 公開日 | 2020-12-29 |
| DOI | 10.1073/pnas.2009931117 |
| PII | 2009931117 |
| PMID | 33318190 |
| PMC | PMC7776818 |
| MeSH | Animals CHO Cells Cell Membrane / metabolism Cricetulus Endocytosis Female Glycosaminoglycans / metabolism* Heparitin Sulfate / metabolism Humans Mutation / genetics Ovarian Neoplasms / metabolism Ovarian Neoplasms / pathology Prions / metabolism* Protein Aggregates* Recombinant Proteins / metabolism Sulfates / metabolism* Tumor Suppressor Protein p53 / chemistry* Tumor Suppressor Protein p53 / genetics Tumor Suppressor Protein p53 / metabolism* |
| IF | 9.412 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | NIH:OVCAR-3(RCB2135) |