論文 - 詳細
| RRC ID | 65160 |
|---|---|
| 著者 | Osakabe K, Wada N, Murakami E, Miyashita N, Osakabe Y. |
| タイトル | Genome editing in mammalian cells using the CRISPR type I-D nuclease. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
Adoption of CRISPR-Cas systems, such as CRISPR-Cas9 and CRISPR-Cas12a, has revolutionized genome engineering in recent years; however, application of genome editing with CRISPR type I-the most abundant CRISPR system in bacteria-remains less developed. Type I systems, such as type I-E, and I-F, comprise the CRISPR-associated complex for antiviral defense ('Cascade': Cas5, Cas6, Cas7, Cas8 and the small subunit) and Cas3, which degrades the target DNA; in contrast, for the sub-type CRISPR-Cas type I-D, which lacks a typical Cas3 nuclease in its CRISPR locus, the mechanism of target DNA degradation remains unknown. Here, we found that Cas10d is a functional nuclease in the type I-D system, performing the role played by Cas3 in other CRISPR-Cas type I systems. The type I-D system can be used for targeted mutagenesis of genomic DNA in human cells, directing both bi-directional long-range deletions and short insertions/deletions. Our findings suggest the CRISPR-Cas type I-D system as a unique effector pathway in CRISPR that can be repurposed for genome engineering in eukaryotic cells. |
| 巻・号 | 49(11) |
| ページ | 6347-6363 |
| 公開日 | 2021-6-21 |
| DOI | 10.1093/nar/gkab348 |
| PII | 6291161 |
| PMID | 34076237 |
| PMC | PMC8216271 |
| MeSH | CRISPR-Associated Proteins / chemistry CRISPR-Associated Proteins / genetics CRISPR-Associated Proteins / metabolism* CRISPR-Cas Systems* Endodeoxyribonucleases / chemistry Endodeoxyribonucleases / genetics Endodeoxyribonucleases / metabolism* Gene Editing* HEK293 Cells Humans Mutagenesis Mutation |
| IF | 11.502 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 11 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293T(RCB2202) |