Reference - Detail
| RRC ID | 6594 |
|---|---|
| Author | Ohta T, Ikemoto Y, Saeki K, Koide T, Wakabayashi S. |
| Title | Histidine-rich glycoprotein and concanavalin A synergistically stimulate the phosphatidylinositol 3-kinase-independent signaling pathway in leukocytes leading to increased cell adhesion and changes in cell morphology. |
| Journal | Cell Immunol |
| Abstract |
Histidine-rich glycoprotein (HRG) promoted the adhesion and morphological changes of human T-cell line MOLT-4 in a Con A-dependent manner. This morphological change-promoting activity was specific for HRG and the Arg23-Lys66 glycopeptide from human HRG. The carbohydrate chain at Asn45 was essential for this activity. The morphological changes of MOLT-4 cells caused by HRG and Con A (HRG/Con A) were not inhibited by phosphatidylinositol 3-kinase inhibitor, wortmannin or LY294002, while the changes by Con A alone were completely inhibited by these reagents, suggesting that HRG/Con A cooperate to activate leukocytes via a signaling pathway distinct from that by Con A alone. The morphological changes by Con A were associated with pseudopodia like structure. On the other hand, the morphological changes caused by HRG/Con A were associated not only with pseudopodia like structure but also with an increase of the F-actin-rich surface protrusions. Wortmannin inhibited only the formation of pseudopodia like structure. |
| Volume | 259(1) |
| Pages | 5-12 |
| Published | 2009-1-1 |
| DOI | 10.1016/j.cellimm.2009.05.001 |
| PII | S0008-8749(09)00080-X |
| PMID | 19535045 |
| MeSH | Androstadienes / pharmacology Anticoagulants / pharmacology Cell Adhesion / drug effects Cell Adhesion / immunology Chromones / pharmacology Concanavalin A / metabolism* Concanavalin A / pharmacology Enzyme Inhibitors / pharmacology Heparin / pharmacology Humans Leukocytes / drug effects Leukocytes / immunology* Mitogens / pharmacology Morpholines / pharmacology Phosphatidylinositol 3-Kinases / metabolism* Phosphoinositide-3 Kinase Inhibitors Protein Kinase Inhibitors / pharmacology Proteins / metabolism* Proteins / pharmacology Pseudopodia / drug effects Pseudopodia / physiology Signal Transduction / drug effects Wortmannin |
| IF | 4.078 |
| Times Cited | 5 |
| WOS Category | IMMUNOLOGY CELL BIOLOGY |
| Resource | |
| Human and Animal Cells | MOLT-4(RCB0206) |