RRC ID 66067
Author Mitsuzawa S, Suzuki N, Akiyama T, Ishikawa M, Sone T, Kawada J, Funayama R, Shirota M, Mitsuhashi H, Morimoto S, Ikeda K, Shijo T, Ohno A, Nakamura N, Ono H, Ono R, Osana S, Nakagawa T, Nishiyama A, Izumi R, Kaneda S, Ikeuchi Y, Nakayama K, Fujii T, Warita H, Okano H, Aoki M.
Title Reduced PHOX2B stability causes axonal growth impairment in motor neurons with TARDBP mutations.
Journal Stem Cell Reports
Abstract Amyotrophic lateral sclerosis (ALS) is an adult-onset incurable motor neuron (MN) disease. The reasons for selective MN vulnerability in ALS are unknown. Axonal pathology is among the earliest signs of ALS. We searched for novel modulatory genes in human MN axon shortening affected by TARDBP mutations. In transcriptome analysis of RNA present in the axon compartment of human-derived induced pluripotent stem cell (iPSC)-derived MNs, PHOX2B (paired-like homeobox protein 2B) showed lower expression in TARDBP mutant axons, which was consistent with axon qPCR and in situ hybridization. PHOX2B mRNA stability was reduced in TARDBP mutant MNs. Furthermore, PHOX2B knockdown reduced neurite length in human MNs. Finally, phox2b knockdown in zebrafish induced short spinal axons and impaired escape response. PHOX2B is known to be highly express in other types of neurons maintained after ALS progression. Collectively, TARDBP mutations induced loss of axonal resilience, which is an important ALS-related phenotype mediated by PHOX2B downregulation.
Volume 16(6)
Pages 1527-1541
Published 2021-6-8
DOI 10.1016/j.stemcr.2021.04.021
PII S2213-6711(21)00216-2
PMID 34048688
PMC PMC8190591
MeSH Amyotrophic Lateral Sclerosis / genetics Amyotrophic Lateral Sclerosis / metabolism* Animals Axons / metabolism* DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Gene Expression Regulation Gene Knockdown Techniques / methods Homeodomain Proteins / genetics Homeodomain Proteins / metabolism* Humans Induced Pluripotent Stem Cells / metabolism* Motor Neurons / metabolism* Mutation Phenotype Transcription Factors / genetics Transcription Factors / metabolism* Transcriptome Zebrafish / metabolism*
IF 6.032
Zebrafish Tg (hb9:Venus) RIKEN WT