論文 - 詳細
| RRC ID | 68106 |
|---|---|
| 著者 | Xu X, Song X, Xu X, Zheng Y, Xu L, Shen L. |
| タイトル | Inhibition of sestrin 1 alleviates polycystic ovary syndrome by decreasing autophagy. |
| ジャーナル | Aging (Albany NY) |
| Abstract |
Polycystic ovary syndrome (PCOS) is the most common endocrine disorder in women of reproductive age, accounting for 50-70% of anovulatory infertility cases. However, the etiology of PCOS at the molecular level remains unclear. Here, bioinformatics analysis was performed to identify differentially expressed genes (DEGs) between adipose tissue of PCOS patients and matched tissues from non-hyperandrogenic women. RT-qPCR, western blot, cell counting kit-8 (CCK-8), EdU (5-Ethynyl-2'-deoxyuridine) staining, LC3 staining, ROS (reactive oxygen species) detection, and apoptosis assays were conducted to explore the effects of sestrin 1 on KGN human granulosa-like tumor cells. Bioinformatics analysis indicated that DEGs in adipose tissue from PCOS patients were enriched in the p53 signaling pathway. Moreover, sestrin 1 was identified as a major target of the p53 gene. Downregulation of sestrin 1 inhibited proliferation of KGN cells by inhibiting autophagy. Additionally, sestrin 1 downregulation increased ROS generation and promoted apoptosis in KGN cells. By contrast, overexpression of sestrin 1 increased cell viability by increasing autophagy in KGN cells. Together, these results suggest that downregulation of sestrin 1 may be a potential novel treatment strategy for PCOS. |
| 巻・号 | 13(8) |
| ページ | 11774-11785 |
| 公開日 | 2021-4-22 |
| DOI | 10.18632/aging.202872 |
| PII | 202872 |
| PMID | 33883304 |
| PMC | PMC8109134 |
| MeSH | Animals Apoptosis / genetics Autophagy / genetics* Case-Control Studies Cell Cycle Proteins / genetics* Cell Line, Tumor Computational Biology Datasets as Topic Disease Models, Animal Female Gene Expression Profiling Gene Knockdown Techniques Granulosa Cells / pathology Healthy Volunteers Heat-Shock Proteins / genetics* Humans Intra-Abdominal Fat / pathology* Omentum Polycystic Ovary Syndrome / genetics* Polycystic Ovary Syndrome / pathology Rats Reactive Oxygen Species Tumor Suppressor Protein p53 / metabolism Up-Regulation |
| IF | 4.831 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | KGN(RCB1154) |