RRC ID 70727
Author Houssin E, Pinot M, Bellec K, Le Borgne R.
Title Par3 cooperates with Sanpodo for the assembly of Notch clusters following asymmetric division of Drosophila sensory organ precursor cells.
Journal Elife
Abstract In multiple cell lineages, Delta-Notch signalling regulates cell fate decisions owing to unidirectional signalling between daughter cells. In Drosophila pupal sensory organ lineage, Notch regulates the intra-lineage pIIa/pIIb fate decision at cytokinesis. Notch and Delta that localise apically and basally at the pIIa-pIIb interface are expressed at low levels and their residence time at the plasma membrane is in the order of minutes. How Delta can effectively interact with Notch to trigger signalling from a large plasma membrane area remains poorly understood. Here, we report that the signalling interface possesses a unique apico-basal polarity with Par3/Bazooka localising in the form of nano-clusters at the apical and basal level. Notch is preferentially targeted to the pIIa-pIIb interface, where it co-clusters with Bazooka and its cofactor Sanpodo. Clusters whose assembly relies on Bazooka and Sanpodo activities are also positive for Neuralized, the E3 ligase required for Delta activity. We propose that the nano-clusters act as snap buttons at the new pIIa-pIIb interface to allow efficient intra-lineage signalling.
Volume 10
Published 2021-10-1
DOI 10.7554/eLife.66659
PII 66659
PMID 34596529
PMC PMC8516416
MeSH Animals Animals, Genetically Modified Cell Division* Cell Lineage Cell Polarity Cytokinesis Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / cytology Drosophila melanogaster / genetics Drosophila melanogaster / metabolism* Gene Expression Regulation, Developmental Intracellular Signaling Peptides and Proteins / genetics Intracellular Signaling Peptides and Proteins / metabolism* Membrane Proteins / genetics Membrane Proteins / metabolism* Microfilament Proteins / genetics Microfilament Proteins / metabolism Receptors, Notch / genetics Receptors, Notch / metabolism* Sense Organs / cytology Sense Organs / metabolism* Signal Transduction Stem Cells / metabolism* Time Factors Ubiquitin-Protein Ligases / genetics Ubiquitin-Protein Ligases / metabolism
IF 7.08
Drosophila DGRC#110685