論文 - 詳細
| RRC ID | 73638 |
|---|---|
| 著者 | Yoshii SR, Kishi C, Ishihara N, Mizushima N. |
| タイトル | Parkin mediates proteasome-dependent protein degradation and rupture of the outer mitochondrial membrane. |
| ジャーナル | J Biol Chem |
| Abstract |
Upon mitochondrial depolarization, Parkin, a Parkinson disease-related E3 ubiquitin ligase, translocates from the cytosol to mitochondria and promotes their degradation by mitophagy, a selective type of autophagy. Here, we report that in addition to mitophagy, Parkin mediates proteasome-dependent degradation of outer membrane proteins such as Tom20, Tom40, Tom70, and Omp25 of depolarized mitochondria. By contrast, degradation of the inner membrane and matrix proteins largely depends on mitophagy. Furthermore, Parkin induces rupture of the outer membrane of depolarized mitochondria, which also depends on proteasomal activity. Upon induction of mitochondrial depolarization, proteasomes are recruited to mitochondria in the perinuclear region. Neither proteasome-dependent degradation of outer membrane proteins nor outer membrane rupture is required for mitophagy. These results suggest that Parkin regulates degradation of outer and inner mitochondrial membrane proteins differently through proteasome- and mitophagy-dependent pathways. |
| 巻・号 | 286(22) |
| ページ | 19630-40 |
| 公開日 | 2011-6-3 |
| DOI | 10.1074/jbc.M110.209338 |
| PII | S0021-9258(20)51041-7 |
| PMID | 21454557 |
| PMC | PMC3103342 |
| MeSH | Animals Cells, Cultured Membrane Proteins / genetics Membrane Proteins / metabolism* Mice Mice, Knockout Mitochondrial Membranes / metabolism* Mitochondrial Proteins / genetics Mitochondrial Proteins / metabolism* Proteasome Endopeptidase Complex / genetics Proteasome Endopeptidase Complex / metabolism* Ubiquitin-Protein Ligases / genetics Ubiquitin-Protein Ligases / metabolism* |
| IF | 4.238 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pMXs-IP-HA-Parkin (RDB19764) pMXs-IP-GFP-Omp25 (RDB19786) |