RRC ID 73640
Author Tamura N, Nishimura T, Sakamaki Y, Koyama-Honda I, Yamamoto H, Mizushima N.
Title Differential requirement for ATG2A domains for localization to autophagic membranes and lipid droplets.
Journal FEBS Lett
Abstract ATG2 is one of the autophagy-related (ATG) proteins essential for autophagosome formation and localizes to isolation membranes and lipid droplets in mammalian cells. Here, we investigated the requirement of regions in ATG2A for its organellar localization and function. The N-terminal amino acids 1-198 and the C-terminal amino acids 1830-1938 are required for the localization to isolation membranes and lipid droplets, respectively. The C-terminal region is not required for the localization to isolation membranes and for autophagy. We also identified an amphipathic helix in ATG2A that is required for both its localization to organelles and autophagosome formation. These data suggest that the dual localization of ATG2A is regulated by different regions.
Volume 591(23)
Pages 3819-3830
Published 2017-12-1
DOI 10.1002/1873-3468.12901
PMID 29113029
MeSH Amino Acid Sequence Animals Autophagosomes / metabolism Autophagosomes / ultrastructure Autophagy / physiology* Autophagy-Related Proteins / chemistry Autophagy-Related Proteins / genetics Autophagy-Related Proteins / metabolism Cells, Cultured Gene Knockout Techniques HEK293 Cells HeLa Cells Humans Lipid Droplets / metabolism Lipid Droplets / ultrastructure Membrane Proteins / chemistry* Membrane Proteins / genetics Membrane Proteins / metabolism* Mice Protein Domains Recombinant Proteins / chemistry Recombinant Proteins / genetics Recombinant Proteins / metabolism Vesicular Transport Proteins / chemistry Vesicular Transport Proteins / genetics Vesicular Transport Proteins / metabolism
IF 3.057
Resource
DNA material pMRXIP-GFP-hATG2A (RDB19782)