RRC ID 75949
Author Doyle JJ, Maios C, Vrancx C, Duhaime S, Chitramuthu B, Bennett HPJ, Bateman A, Parker JA.
Title Chemical and genetic rescue of in vivo progranulin-deficient lysosomal and autophagic defects.
Journal Proc Natl Acad Sci U S A
Abstract In 2006, GRN mutations were first linked to frontotemporal dementia (FTD), the leading cause of non-Alzheimer dementias. While much research has been dedicated to understanding the genetic causes of the disease, our understanding of the mechanistic impacts of GRN deficiency has only recently begun to take shape. With no known cure or treatment available for GRN-related FTD, there is a growing need to rapidly advance genetic and/or small-molecule therapeutics for this disease. This issue is complicated by the fact that, while lysosomal dysfunction seems to be a key driver of pathology, the mechanisms linking a loss of GRN to a pathogenic state remain unclear. In our attempt to address these key issues, we have turned to the nematode, Caenorhabditis elegans, to model, study, and find potential therapies for GRN-deficient FTD. First, we show that the loss of the nematode GRN ortholog, pgrn-1, results in several behavioral and molecular defects, including lysosomal dysfunction and defects in autophagic flux. Our investigations implicate the sphingolipid metabolic pathway in the regulation of many of the in vivo defects associated with pgrn-1 loss. Finally, we utilized these nematodes as an in vivo tool for high-throughput drug screening and identified two small molecules with potential therapeutic applications against GRN/pgrn-1 deficiency. These compounds reverse the biochemical, cellular, and functional phenotypes of GRN deficiency. Together, our results open avenues for mechanistic and therapeutic research into the outcomes of GRN-related neurodegeneration, both genetic and molecular.
Volume 118(25)
Published 2021-6-22
DOI 10.1073/pnas.2022115118
PII 2022115118
PMID 34140407
PMC PMC8237633
MeSH Acetophenones / pharmacology Animals Autophagy / genetics* Benzopyrans / pharmacology Biosynthetic Pathways Caenorhabditis elegans / cytology Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Drug Evaluation, Preclinical Frontotemporal Dementia / genetics Frontotemporal Dementia / pathology Lysosomes / genetics* Mutation / genetics Phenotype Progranulins / genetics Progranulins / metabolism* Rivastigmine / pharmacology Small Molecule Libraries / pharmacology Sphingolipids / metabolism
Resource
C.elegans tm985