論文 - 詳細
| RRC ID | 76015 |
|---|---|
| 著者 | Montgomery BE, Vijayasarathy T, Marks TN, Cialek CA, Reed KJ, Montgomery TA. |
| タイトル | Dual roles for piRNAs in promoting and preventing gene silencing in C. elegans. |
| ジャーナル | Cell Rep |
| Abstract |
Piwi-interacting RNAs (piRNAs) regulate many biological processes through mechanisms that are not fully understood. In Caenorhabditis elegans, piRNAs intersect the endogenous RNA interference (RNAi) pathway, involving a distinct class of small RNAs called 22G-RNAs, to regulate gene expression in the germline. In the absence of piRNAs, 22G-RNA production from many genes is reduced, pointing to a role for piRNAs in facilitating endogenous RNAi. Here, however, we show that many genes gain, rather than lose, 22G-RNAs in the absence of piRNAs, which is in some instances coincident with RNA silencing. Aberrant 22G-RNA production is somewhat stochastic but once established can occur within a population for at least 50 generations. Thus, piRNAs both promote and suppress 22G-RNA production and gene silencing. rRNAs and histones are hypersusceptible to aberrant silencing, but we do not find evidence that their misexpression is the primary cause of the transgenerational sterility observed in piRNA-defective mutants. |
| 巻・号 | 37(10) |
| ページ | 110101 |
| 公開日 | 2021-12-7 |
| DOI | 10.1016/j.celrep.2021.110101 |
| PII | S2211-1247(21)01595-3 |
| PMID | 34879267 |
| PMC | PMC8730336 |
| MeSH | Animals Animals, Genetically Modified Argonaute Proteins / genetics* Argonaute Proteins / metabolism Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Histones / genetics Histones / metabolism Models, Genetic Mutation* RNA Interference* RNA, Helminth / genetics* RNA, Helminth / metabolism RNA, Ribosomal / genetics* RNA, Ribosomal / metabolism RNA, Small Interfering / genetics* RNA, Small Interfering / metabolism Stochastic Processes Transcription, Genetic* |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm872 tm1301 |