RRC ID 76015
Author Montgomery BE, Vijayasarathy T, Marks TN, Cialek CA, Reed KJ, Montgomery TA.
Title Dual roles for piRNAs in promoting and preventing gene silencing in C. elegans.
Journal Cell Rep
Abstract Piwi-interacting RNAs (piRNAs) regulate many biological processes through mechanisms that are not fully understood. In Caenorhabditis elegans, piRNAs intersect the endogenous RNA interference (RNAi) pathway, involving a distinct class of small RNAs called 22G-RNAs, to regulate gene expression in the germline. In the absence of piRNAs, 22G-RNA production from many genes is reduced, pointing to a role for piRNAs in facilitating endogenous RNAi. Here, however, we show that many genes gain, rather than lose, 22G-RNAs in the absence of piRNAs, which is in some instances coincident with RNA silencing. Aberrant 22G-RNA production is somewhat stochastic but once established can occur within a population for at least 50 generations. Thus, piRNAs both promote and suppress 22G-RNA production and gene silencing. rRNAs and histones are hypersusceptible to aberrant silencing, but we do not find evidence that their misexpression is the primary cause of the transgenerational sterility observed in piRNA-defective mutants.
Volume 37(10)
Pages 110101
Published 2021-12-7
DOI 10.1016/j.celrep.2021.110101
PII S2211-1247(21)01595-3
PMID 34879267
PMC PMC8730336
MeSH Animals Animals, Genetically Modified Argonaute Proteins / genetics* Argonaute Proteins / metabolism Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Histones / genetics Histones / metabolism Models, Genetic Mutation* RNA Interference* RNA, Helminth / genetics* RNA, Helminth / metabolism RNA, Ribosomal / genetics* RNA, Ribosomal / metabolism RNA, Small Interfering / genetics* RNA, Small Interfering / metabolism Stochastic Processes Transcription, Genetic*
C.elegans tm872 tm1301