Reference - Detail
| RRC ID | 76036 |
|---|---|
| Author | Puri D, Ponniah K, Biswas K, Basu A, Dey S, Lundquist EA, Ghosh-Roy A. |
| Title | Wnt signaling establishes the microtubule polarity in neurons through regulation of Kinesin-13. |
| Journal | J Cell Biol |
| Abstract |
Neuronal polarization is facilitated by the formation of axons with parallel arrays of plus-end-out and dendrites with the nonuniform orientation of microtubules. In C. elegans, the posterior lateral microtubule (PLM) neuron is bipolar with its two processes growing along the anterior-posterior axis under the guidance of Wnt signaling. Here we found that loss of the Kinesin-13 family microtubule-depolymerizing enzyme KLP-7 led to the ectopic extension of axon-like processes from the PLM cell body. Live imaging of the microtubules and axonal transport revealed mixed polarity of the microtubules in the short posterior process, which is dependent on both KLP-7 and the minus-end binding protein PTRN-1. KLP-7 is positively regulated in the posterior process by planar cell polarity components of Wnt involving rho-1/rock to induce mixed polarity of microtubules, whereas it is negatively regulated in the anterior process by the unc-73/ced-10 cascade to establish a uniform microtubule polarity. Our work elucidates how evolutionarily conserved Wnt signaling establishes the microtubule polarity in neurons through Kinesin-13. |
| Volume | 220(9) |
| Published | 2021-9-6 |
| DOI | 10.1083/jcb.202005080 |
| PII | 212396 |
| PMID | 34137792 |
| PMC | PMC8217938 |
| MeSH | Animals Axons / metabolism Axons / ultrastructure Biological Transport Caenorhabditis elegans / cytology Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Cell Differentiation Cell Polarity / genetics Dendrites / metabolism Dendrites / ultrastructure Gene Expression Regulation Kinesins / genetics* Kinesins / metabolism Microtubule-Associated Proteins / genetics* Microtubule-Associated Proteins / metabolism Microtubules / metabolism* Microtubules / ultrastructure Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism Neurogenesis / genetics* Wnt Signaling Pathway / genetics* rac GTP-Binding Proteins / genetics rac GTP-Binding Proteins / metabolism rho GTP-Binding Proteins / genetics rho GTP-Binding Proteins / metabolism rho-Associated Kinases / genetics rho-Associated Kinases / metabolism |
| Resource | |
| C.elegans | tm2143 |