論文 - 詳細
| RRC ID | 76068 |
|---|---|
| 著者 | Sun J, Harion R, Naito T, Saheki Y. |
| タイトル | INPP5K and Atlastin-1 maintain the nonuniform distribution of ER-plasma membrane contacts in neurons. |
| ジャーナル | Life Sci Alliance |
| Abstract |
In neurons, the ER extends throughout all cellular processes, forming multiple contacts with the plasma membrane (PM) to fine-tune neuronal physiology. However, the mechanisms that regulate the distribution of neuronal ER-PM contacts are not known. Here, we used the Caenorhabditis elegans DA9 motor neuron as our model system and found that neuronal ER-PM contacts are enriched in soma and dendrite and mostly absent in axons. Using forward genetic screen, we identified that the inositol 5-phosphatase, CIL-1 (human INPP5K), and the dynamin-like GTPase, ATLN-1 (human Atlastin-1), help to maintain the non-uniform, somatodendritic enrichment of neuronal ER-PM contacts. Mechanistically, CIL-1 acts upstream of ATLN-1 to maintain the balance between ER tubules and sheets. In mutants of CIL-1 or ATLN-1, ER sheets expand and invade into the axon. This is accompanied by the ectopic formation of axonal ER-PM contacts and defects in axon regeneration following laser-induced axotomy. As INPP5K and Atlastin-1 have been linked to neurological disorders, the unique distribution of neuronal ER-PM contacts maintained by these proteins may support neuronal resilience during the onset and progression of these diseases. |
| 巻・号 | 4(11) |
| 公開日 | 2021-11-1 |
| DOI | 10.26508/lsa.202101092 |
| PII | 4/11/e202101092 |
| PMID | 34556534 |
| PMC | PMC8507493 |
| MeSH | Animals Axons / metabolism Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / metabolism Cell Membrane / metabolism Endoplasmic Reticulum / metabolism GTP Phosphohydrolases / metabolism GTP-Binding Proteins / metabolism* Humans Membrane Proteins / metabolism* Membrane Proteins / physiology Microtubules / metabolism Motor Neurons / metabolism* Motor Neurons / physiology Nerve Regeneration Neurons / metabolism Phosphoric Monoester Hydrolases / metabolism* Phosphoric Monoester Hydrolases / physiology |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 15 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm390 tm14710 tm3568 FX30203 |