| RRC ID |
77370
|
| Author |
Ueda T, Hanai A, Takei T, Kubo K, Ohgi M, Sakagami H, Takahashi S, Shin HW, Nakayama K.
|
| Title |
EFA6 activates Arf6 and participates in its targeting to the Flemming body during cytokinesis.
|
| Journal |
FEBS Lett
|
| Abstract |
The small GTPase Arf6 is transiently associated with the ingressing cleavage furrow and subsequently targeted to the Flemming body during cytokinesis, suggesting its activation around the cleavage furrow. Here, we show that EFA6 (exchange factor for Arf6) localizes on the cleavage furrow through its PH domain. Time-lapse analysis showed that both EFA6 and Arf6 are transiently localized around the ingressing cleavage furrow, but only Arf6 is subsequently targeted to the Flemming body. Expression of an EFA6 mutant suppresses Arf6 recruitment onto the Flemming body. These results suggest that EFA6 participates in activation of Arf6 around the cleavage furrow during cytokinesis.
|
| Volume |
587(11)
|
| Pages |
1617-23
|
| Published |
2013-6-5
|
| DOI |
10.1016/j.febslet.2013.03.042
|
| PII |
S0014-5793(13)00298-6
|
| PMID |
23603394
|
| MeSH |
ADP-Ribosylation Factor 6
ADP-Ribosylation Factors / metabolism*
Amino Acid Sequence
Amino Acid Substitution
Conserved Sequence
Cytokinesis*
Enzyme Activation
Guanine Nucleotide Exchange Factors
HeLa Cells
Humans
Microscopy, Fluorescence
Mutagenesis, Site-Directed
Nerve Tissue Proteins / chemistry
Nerve Tissue Proteins / genetics
Nerve Tissue Proteins / metabolism*
Protein Structure, Tertiary
Protein Transport
Single-Cell Analysis
Time-Lapse Imaging
|
| IF |
3.057
|
| Resource |
| DNA material |
EGFP-hEFA6A (RDB20108)
EGFP-hEFA6B (RDB20109)
EGFP-hEFA6C (RDB20110) |
